trxS基因对大麦发芽籽粒中蛋白质降解的影响(英文)  

Effects of trxS gene on protein degradation in germinating barley seeds

作　　者：卫丽[1] 孔维威[2] 尹钧[1] 王伟杰[1] 

机构地区：[1]河南农业大学,郑州450002 [2]南京农业大学生命科学院,南京210095

出　　处：《生物工程学报》2009年第1期84-88,共5页Chinese Journal of Biotechnology

摘　　要：对转trxS基因大麦籽粒发芽过程中蛋白酶活性、不同蛋白组分含量和贮藏蛋白SDS-PAGE图谱的变化进行了研究。结果表明:与对照相比,转基因籽粒中的蛋白酶活性提高;清蛋白、球蛋白、醇溶蛋白和谷蛋白含量低于对照。SDS-PAGE图谱也表明,转基因籽粒中贮藏蛋白降解快于对照。We assessed the effects of trxS gene on changes of proteinase activity, contents of different protein fractions and SDS-PAGE profiles in germinating seeds of contrasting transgenic and nontransgenic barley variety. Proteinase activity was enhanced by 70.28% in transgenic than nontransgenic barley seeds, whereas contents of albumin, globulin, hordein and glutelin in transgenic seeds were 3.68%, 23.52%, 31.37%, and 21.04%, lower than those in nontransgenic seeds. Degradation rates of hordein and glutelin in transgenic seeds were faster than those in nontransgenic seedlings as indicated by the SDS-PAGE profiles. Our data imply that the l/ansformation of trxS gene could promote the degradation of protein, providing theoretic basis for the use of trxS gene and barley quality breeding.

关 键 词：TRXS基因 蛋白质 蛋白酶 SDS—PAGE 

分 类 号：S512.3[农业科学—作物学] TS252.53[轻工技术与工程—农产品加工及贮藏工程]