HG颗粒对肝纤维化大鼠GSH、MDA及TNF-α含量的影响  

Effects of HG Keli on GSH,MDA and TNF-α of HF model rats induced by Carbon Tetrar-chloride compounded other factors

作　　者：毕红征[1] 杜春燕[2] 黄国钧[3] 王纯耀[2] 

机构地区：[1]郑州大学基础医学院药理学教研室,郑州450001 [2]郑州大学实验动物中心,郑州450052 [3]成都中医药大学药学院,成都610075

出　　处：《郑州大学学报（医学版）》2009年第1期169-171,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究HG颗粒对CCl4所致肝纤维化大鼠肝脏谷胱甘肽(GSH)、丙二醛(MDA)及血清肿瘤坏死因子-α(TNF-α)含量的影响。方法:80只SD大鼠随机分为6组,除正常对照组10只外,其余各组大鼠皮下注射CCl4,同时给予高脂饲料和体积分数为20%乙醇喂养6周,建立肝纤维化大鼠模型,存活动物随机分为5组,正常对照组(蒸馏水1mL/kg),模型对照组(蒸馏水1mL/kg),HG颗粒高、中、低3种剂量组(6、3、1.5g/kgHG),复方鳖甲软肝片组(1g/kg复方鳖甲软肝片)。6组动物1次/d灌胃,连续4周。末次给药24h后处死动物,观察各组大鼠肝脏GSH、MDA及血清TNF-α含量的变化。结果:模型对照组大鼠肝脏GSH、MDA及血清TNF-α含量[(5.01±1.92)μg/g,(230.4±29.47)μg/g,(2.71±0.62)nmol/L]与正常对照组[(9.65±2.31)μg/g,(101.7±13.79)μg/g,(0.72±0.15)nmol/L]比较出现明显异常,HG颗粒可显著改善肝纤维化大鼠各观察指标的异常。结论:HG颗粒对CCl4致的大鼠肝纤维化有良好的治疗作用。Aim ： To explore the effects of HG keli on GSH and MDA in the liver and TNF-α in the serum of hepatic fibrosis（HF） models rats. Methods： Eighty SD rats were divided into six groups. Excluding ten rats in normal control group, the other rats were injected carbon tetrar-chloride hypodermicly,simultaneously with high fat feed and 20% ethyl alcohol for six weeks to establish HF model rats. Existing rats were divided into five groups basing on sex and weight, groups added up to six goups including normal control group. All rats were given drugs or distilled water. Normal control group： distilled water I mL/kg ; model control group ： distilled water 1 mL/kg; HG Keli high, middle, low groups ： 6 g, 3 g, 1.5 g crude drug/kg;FFBJRG tablet group： 1 g/kg. Trended the HF rats with drugs through tube feeding once a day for four weeks. Observed the contents of GSH and MDA in the liver and TNF-α in the serum. Results： GSH and MDA in the liver and TNF-α in the serum in model mice were significantly abnormal compared with normal group. HG Keli meliorated them obviously. Conclusion ： These results suggested HG Keli can effectively cure HF model rats.

关 键 词：HG颗粒 肝纤维化 谷胱甘肽 丙二醛 肿瘤坏死因子-α 大鼠 

分 类 号：R285.5[医药卫生—中药学]