N-乙酰半胱氨酸对糖尿病大鼠脾细胞凋亡及免疫相关因子的干预作用  

Intervention of N-acetylcysteine on splenocyte apoptosis and immune associated factors in rats with diabetes mellitus

作　　者：郭彩霞[1] 王志成[2] 龚平生[3] 李艳博[2] 赵红光[2] 郭伟[2] 龚守良[2] 

机构地区：[1]吉林大学公共卫生学院卫生毒理学教研室,吉林长春130021 [2]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [3]吉林大学分子酶学工程教育部重点实验室,吉林长春130023

出　　处：《吉林大学学报（医学版）》2009年第1期1-4,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(39870312)

摘　　要：目的:观察N-乙酰半胱氨酸(NAC)对糖尿病(DM)大鼠脾细胞凋亡和免疫因子的影响,为NAC在糖尿病并发症预防及糖尿病的治疗方面提供理论依据。方法:实验分为对照组、DM组和NAC+DM组,链脲佐菌素腹腔注射诱导DM大鼠模型,灌胃给予NAC,于给药结束4周末断头处死,分别采用Annexin V/碘化丙啶(PI)双荧光标记、PI单标记和PE标记TCRαβ抗体标记,流式细胞术检测脾细胞凋亡、细胞周期及TCRαβ百分数变化;ELISA法检测血清和脾细胞培养上清IL-2含量的变化。结果:与对照组比较,DM组大鼠脾细胞凋亡率、TCRαβ百分数及血清IL-2含量、G0/G1期和G2/M期脾细胞数明显升高(P<0.05或P<0.001),S期脾细胞数和培养上清IL-2含量明显降低(P<0.05或P<0.001);DM+NAC组脾细胞凋亡率有所下降,与对照组比较差异无显著性,且细胞周期各期细胞数无明显变化。与DM组比较,DM+NAC组脾细胞凋亡率、TCRαβ百分数、血清IL-2含量、G0/G1期和G2/M期脾细胞数明显降低(P<0.05或P<0.001),S期脾细胞数和培养上清IL-2含量明显升高(P<0.05)。结论:NAC能降低DM所致的脾细胞凋亡增加,可纠正DM引发的脾细胞周期阻滞,调节DM所致的免疫因子失衡。Objective To explore the effect of N-acetylcysteine （NAC） on splenocyte apoptosis and immune associated factors in rats with diabetes mellitus DM）, to provide the theoretic evidence for prevention of the complication of DM and therapy of DM. Methods The rats were divided into control group, DM group and NAC ＋DM group. The DM rat model was induced by mtraperitoneal injection of streptozotocin. At the fourth weekend after giving NAC through intragastric administration, the apoptosis, cell cycle and TCRαβ of splenocytes were detected with flow cytometry, Annexin V/PI double-staining, PI single-labeling and TCRαβ antibody-PE respectively; and the IL-2 contents in serum and splenocyte culture supernatant were measured with ELISA. Results As compared with control group, the apoptotic rate, TCRαβ of splenocytes and the serum IL-2 content in rats in DM group increased significantly （P〈0. 001）, and both the percentage of splenoeytes in G0/G1 and G2/M phases increased significantly （P〈0.05）, and the percentage of splenoeytes in S phase and the IL-2 content in splenocyte culture supernatant decreased significantly （P〈0.05）; the apoptosis of splenocytes did not decrease significantly, and so was that of cell cycle. As compared with DM group, the apoptotic rate, TCRαβ of splenocytes and the serum IL-2 content in DM ＋ NAC group decreased significantly （P 〈 0. 001 ）, and the percentages of splenocytes in both G0/G1 and G2/M phases decreased significantly （P〈0.05）, and that in S phase and IL-2 content in splenoeyte culture supernatant increased significantly （P〈 0.05）. Conclusion NAC could decrease the increase of splenocyte apoptosis rectify the cell cycle delay of splenocytes and regulate the immune factor imbalance induced by DM.

关 键 词：N-乙酰半胱氨酸 糖尿病 脾细胞 细胞凋亡 细胞周期 

分 类 号：R587.1[医药卫生—内分泌] R392[医药卫生—内科学]