褪黑素、维生素E对阿霉素所致大鼠心肌损伤的影响  

Effects of melatonin and VitE on adriamycin induced myocardial injury in rats

作　　者：林海[1] 唐戍平[1] 

机构地区：[1]安徽医科大学附属省立医院安徽省立医院老年病科,合肥230001

出　　处：《中国临床保健杂志》2009年第1期48-50,114,共4页Chinese Journal of Clinical Healthcare

摘　　要：目的研究褪黑素(MLT)、维生素(Vit)E对阿霉素(ADM)导致大鼠心肌损伤的影响。方法采用成年SD大鼠40只,随机分为空白对照组、ADM组、MLT+ADM组、VitE+ADM组、MLT+VitE+ADM组,每组各8只。实验30 d后Masson氏三色染色法进行左室胶原特异染色及半定量分析,计算胶原容积分数(CVF),并作HE染色,光镜下病理形态学观察,测定心肌超氧化物酶(SOD)活性及丙二醛(MDA)含量,测定Bcl-2/Bax蛋白的表达。结果与正常对照组大鼠比较,阿霉素组大鼠心肌MDA含量显著升高,而SOD活性显著降低,心肌细胞Bax/Bcl-2基因的蛋白阳性表达增高,心肌损伤。给予MLT、VitE治疗可明显降低心肌组织中MDA含量,并显著增强SOD活性,抑制心肌细胞Bcl-2/Bax基因的蛋白阳性表达心肌损伤减轻。2种抗氧化剂相比,MLT在大鼠心肌组织的抗氧化能力强于VitE,两者联用则抗氧化能力强于两者单独应用。结论MLT和VitE均可通过增强阿霉素诱导的心肌损伤大鼠的抗氧化能力和抑制氧化应激反应从而对心肌具有一定的保护作用,并且MLT的抗氧化能力强于VitE,联用MLT和VitE则抗氧化能力表现更明显。Objective To study effects of melatonin and VitE on adriamycin induced myocardial injury in rats. Methods Forty adult SD rats were randomized into five groups （ n = 8 ） ： control group, ADM group, MLT ＋ ADM group,VitE ＋ ADM group,MLT ＋ VitE ＋ ADM group. After 30 days,left ventricular collagen volume fraction （CVF） ,the activity of myocardial superoxide dismutase （SOD）, the concentration of malondialdehyde （MDA）, the Bax/Bcl-2 of protein expression were assayed. The pathomorphology changes cardiomyocytes were detected optical microscope. Results Compared with the control group,the concentration of MDA in ADM group rats were significantly higher, the activity of SOD were significantly lower, myocardial cells＇ Bax gene protein expression were higher and Bcl-2 gene protein expression were lower. MLT and VitE treatment can significantly reduce the myocardial tissue＇ concentration of MDA, and enhance the activity of SOD and the expression of bcl-2 increased while the expression of bax decreased significantly. Compared to the two kinds of antioxidants, MLT of the antioxidant capacity was higher than VitE, the combined effect of antioxidant capacity was more than the single one. Conclusion MLT and VitE can enhance myocardial antioxidant capacity and protection aganist myocardial injures, the combined effect of antioxidant capacity is significantly, the mechanisms are possibly due to MLT and VitE can prevent free radical damage from various sectors.

关 键 词：心肌疾病 抗生素类 抗肿瘤/副作用 褪黑激素 维生素E 大鼠 

分 类 号：R96[医药卫生—药理学]