尾加压素Ⅱ对培养乳鼠心肌细胞内钙离子的影响及其机制  

The effect of urontensin Ⅱ on intracellular calcium concentration of cardiomyocytes in neonatal rat

作　　者：李巍[1] 李爱民[1] 

机构地区：[1]第三军医大学新桥医院心内科全军心血管内科研究所,重庆400037

出　　处：《西部医学》2009年第2期175-177,共3页Medical Journal of West China

基　　金：重庆市自然基金项目(NO:CSTC2007BB5026)

摘　　要：目的观察尾加压素Ⅱ(UⅡ)对体外培养乳鼠心肌细胞内钙离子浓度的影响。方法把体外培养的SD乳鼠心肌细胞以Fluo3/AM荧光指示剂负载,分为4组:正常对照组;单纯UⅡ干预组;尼卡地平提前阻断+UⅡ干预组;IP3受体拮抗剂Xestspongin C+UⅡ干预组。应用激光共聚焦显微镜技术检测心肌细胞钙离子浓度变化。结果正常对照组心肌细胞内钙离子荧光强度较低(31.65±2.37);单纯UⅡ干预组钙离子荧光强度增加明显(87.13±5.72);尼卡地平提前阻断+UⅡ干预组以及IP3受体拮抗剂Xestspongin C+UⅡ干预组钙离子也有增加,分别为(59.43±4.76)、(54.79±5.18)。结论UⅡ可引起心肌细胞内钙离子浓度增高,其主要的机制可能与细胞外钙离子内流和肌浆网钙离子释放增加有关。Objective To observe the effect of urotensin Ⅱ （U Ⅱ） on the intracellular calcium concentration of cardiomyocytes in neonatal rat. Methods The cardiomyocytes of neonatal rat were cultured in vitro and treated with UⅡ. The concentration of intracellular calcium was observed with laser scanning confocal microscope（LSCM）. Results The concentration of Ca^2＋ in cardiomyocytes was increased shortly after treatment with UⅡ, which was partly abolished before treated with nicardipine and xestspongin C. Conclusion UⅡ can increase the concentration of calcium in cardiomyocytes.

关 键 词：尾加压素Ⅱ 心肌细胞 钙 激光共聚焦显微镜技术 

分 类 号：R54[医药卫生—心血管疾病]