甘肃棘豆中产苦马豆素内生真菌的分离与鉴定  

Isolation and identification of swainsonine-producing fungal endophyte from Oxytropis kansuensis

作　　者：余永涛[1] 王建华[1] 赵清梅[2] 李海利[1] 宋毓民[1] 王妍[1] 张志敏[1] 崔忠华[1] 耿果霞[1] 李勤凡[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]北方民族大学生命科学与工程学院,宁夏银川750021

出　　处：《西北农林科技大学学报（自然科学版）》2009年第2期40-46,51,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30871901,30571315)

摘　　要：【目的】确定甘肃棘豆中是否存在产生苦马豆素(SW)的内生真菌。【方法】对甘肃棘豆的内生真菌进行分离,应用薄层色谱法和气相色谱-甲基化--αD-甘露糖苷内标法分别对菌丝中SW进行检测,筛选可生成SW的疯草内生真菌;通过形态学观察,应用聚合酶链反应对5.8S rDNA-ITS序列进行扩增,并对扩增序列进行分析,构建系统发育树,对其进行种属分类。【结果】筛选出1种可以产生SW的甘肃棘豆内生真菌FEL3,气相色谱内标法测得其菌丝中SW含量为400.52μg/g。根据形态学、5.8S rDNA-ITS序列分析结果和文献报道,确定FEL3为埃里格孢属真菌。【结论】甘肃棘豆中存在生成SW的内生真菌Embellisiasp.FEL3。[Objective] The study was to confirm whether Oxytropis kansuensis is infected by the swainsonine producing fungal endophyte. [Method] One of the main locoweed in China was assessed for endophytic fungi. The chemical composition of isolated fungus were detected by thin-layer chromatography and gas chromatography-methyl-α-D-mannopyranoside internal standard method. In addition to observing the morphology of swainsonine-producing fungal endophyte,we analysed the 5.8S rDNA-ITS sequence of the fungi that was amplified by polymerase chain reaction. Depending on the analyzed result of the sequence,the phylogenetic tree was established based on the morphology, the phylogenetic tree and documents. [Result] A species of fungal endophyte that producing swainsonine, FEL3, was determined. FEL3 was identified as Embellisia sp. FEL3. [Conclusion] This is the first report about swainsonine-producing fungal endophyte from Oxytropis kansuensis.

关 键 词：甘肃棘豆 苦马豆素 内生真菌 埃里格孢属 

分 类 号：Q949.32[生物学—植物学]