冬枣组织培养的消毒方法  

Sterilized Methods on Tissue Culture of Winter Ziziphus Jujuba

作　　者：刘雪红[1] 刘俊华[1] 张孝霖[1] 

机构地区：[1]滨州学院生命科学系,山东滨州256603

出　　处：《北方园艺》2009年第2期97-98,共2页Northern Horticulture

基　　金：滨州学院自然科学基金资助项目(BZXYQNLG2005015)

摘　　要：为了优化冬枣组织培养的消毒方法，比较分析了乙醇、升汞、次氯酸钠和吐温等4种消毒剂对冬枣茎尖、叶片、茎段以及叶柄等组织培养的消毒效果。结果表明：70％乙醇30s，0.1％升汞加3-5滴吐温20消毒6win既能减少污染，又能提高外植体的成活率，综合效果最佳。Effects of sterilization on explants and growth on callus were studied in the stem tip, lamina, stem segment and leafstalk tissue culture of winter Ziziphus jujuba by means of 70% alcohol, mercuric chloride, sodium hypochlorite and tween-20 in order to establish the sterilization method of the tissue culture. The result indicated that 70% alcohol sterilized the explants for 30 seconds, 0.1% mercuric chloride with 3-5 drops of tween-20 sterilized the explants for six minutes, which not only decreased the explants pollution rate, but also promoted the survival rate of the explants.

关 键 词：冬枣 组织培养 消毒方法 

分 类 号：S665.103[农业科学—果树学]