菘蓝试管苗练苗及移栽技术研究  

Study on Domestication and Transplanting Technique of Tissue Culture Plantlets of Isatis Indigotica Fort.

作　　者：张胜珍[1] 客绍英[1] 孟文霞[1] 杨飞飞[1] 

机构地区：[1]唐山师范学院生命科学系,河北唐山063000

出　　处：《北方园艺》2009年第2期237-240,共4页Northern Horticulture

基　　金：河北省科技攻关项目(05276416);唐山市科技攻关项目(03132001A)

摘　　要：以菘蓝试管苗为试验材料,研究了练苗时间、移栽基质、营养液等一系列因子对菘蓝试管苗移栽成活率及生长状况的影响。结果表明:菘蓝试管苗最佳练苗时间为4 d。以蛭石∶珍珠岩=1∶2为移栽基质时,试管苗成活率最高(达97.5%),长势最佳。用大量元素为Ca(NO3)2945 mg/L+KNO3607 mg/L+(NH4)2HPO4115 mg/L+MgSO4493 mg/L的营养液配方浇灌菘蓝移栽幼苗,可明显促进移栽苗的生长。The tissue culture plantlets of Isatis indigotica Fort. were used as experiment materials and the influences of domestication time, transplanting medium and nutrition liquid combinations on the survival rate and growth of Isatis indigotica Fort. were studied. The results showed that the optimum domestication time was three days. The plantlets transplanted to the substrate with vermiculite and perlite（1：2） gained the highest survival rate and the best condition of growth. The growth of plantlets of Isatis indigotica Fort. can be promoted obviously using prescription of nutrition liquid whose vast elements were Ca（NO3）2 945mg/L ＋ KNO3 607mg/L＋（NH4）2 HPO4 115mg/L＋MgSO4 493mg/L.

关 键 词：菘蓝 试管苗 练苗 移栽 

分 类 号：S567.9[农业科学—中草药栽培]