鸡毒支原体PCR检测方法的建立  

Establishment of PCR for Detection of Mycoplasma gallisepticum

作　　者：秦璐璐[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《动物医学进展》2009年第2期39-42,共4页Progress In Veterinary Medicine

摘　　要：根据已发表的鸡毒支原体种特异性序列fMG-2设计1对引物,建立检测鸡毒支原体的PCR方法。该法对鸡毒支原体能特异性扩增726 bp的目的片段,而对其他禽病原DNA模板的扩增结果为阴性。建立的PCR方法对鸡毒支原体的最少检出量为3 pg。用建立的PCR方法对临床采集的样品进行检测,同时对相应的样品进行细菌分离,结果临床样品PCR的阳性检出率为20.5%,细菌分离培养的阳性率为0.9%,表明PCR的敏感性高于细菌分离鉴定。A pair of primers was designed based on fMG-2 sequences of avian Mycoplasma. The primers selectively amplified a 726 bp product from Mycoplasrna gallisepticurn （MG）, but did not amplify the DNA or RNA of other bacteria or viruses. The detection threshold of PCR for MG was 3 pg. The samples detected by PCR,and isolation and identification. Using PCR,20.5% of field samples was positively detected, it was higher than that of isolation and identification. The results indicated that this technique is a useful screening assay for the detection of MG.

关 键 词：鸡毒支原体 聚合酶链反应 检测 分离鉴定 

分 类 号：S852.62[农业科学—基础兽医学]