蔓花生组织培养和植株再生的条件优化  

Condition optimization for tissue culture of Arachis duranensis

作　　者：张振霞[1] 郑玉忠[1] 

机构地区：[1]韩山师范学院生物系,广东潮州521041

出　　处：《广西植物》2009年第1期83-86,95,共5页Guihaia

基　　金：国家星火计划项目(2005EA780080);韩山师范学院青年基金~~

摘　　要：应用MS为基本培养基,通过各种培养条件和不同激素配比,探讨蔓花生组织培养及其植株再生条件的优化。结果显示:幼叶为最佳的外植体,在幼叶愈伤诱导过程中,不超过12h光照,光照强度在21.6μmol.m-2.s-1均可;诱导愈伤组织的适宜培养基为MS+0.5mg/L6-BA+0.2mg/L2,4-D或MS+0.5mg/L6-BA+2mg/LNAA;最适的分化培养基为MS+1mg/LTDZ+2mg/L6-BA+0.5mg/LNAA;最适的生根培养基1/2MS+1mg/LNAA+1mg/LPP333。Condition optimization for tissue culture and regeneration of Arachis duranensis was studied. The results showed that the leaflet was the best experimental explant;the optimized medium for callus was MS＋0.5 mg/L6-BA ＋0.2 mg/L2,4-D or MS＋0.5 mg/L6-BA＋0.2 mg/LNAA with illumination of 12h/d; the optimized medium for differentiation was MS＋ 1 mg/LTDZ-2 mg/L6-BA＋0.5 mg/LNAA; the optimized medium for root induction was 1/2MS＋ 1 mg/LNAA＋ 1 mg/LPP333.

关 键 词：蔓花生 组织培养 愈伤组织 

分 类 号：Q943.1[生物学—植物学]