匙羹藤组织培养条件优化研究  

Optimization in vitro culture conditions for Gymnema sylvestre

作　　者：苏钛[1,2] 黄宁珍[1] 付传明[1] 

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西桂林541006 [2]广西师范大学生命科学学院,广西桂林541004

出　　处：《广西植物》2009年第1期87-91,共5页Guihaia

基　　金：广西科技攻关项目(桂科攻0330003-1,0424008-1H)~~

摘　　要：以匙羹藤无菌种子苗为外植体,通过预备实验确定基本培养基后,采用完全实验和正交设计相结合的方法,研究植物生长调节剂两因素配合和多因素组合(NAA、TDZ、2-ip、IAA、6-BA和KT)对匙羹藤芽体诱导及继代培养的影响。结果显示,匙羹藤组织培养最适的基本培养基为N68;N68+NAA0.05mg/L+2-ip0.4mg/L+TDZ0.001mg/L利于芽体增殖,可用于继代培养;N68+NAA0.05mg/L+TDZ0.005mg/L促进植株长根,可用于生根培养。The optimum medium for tissue culture of Oymnema sylvestre were studied by using germfree tubseedling as explants. The effects of plant growth regulator （NAA, TDZ, 2-ip, IAA, 6-BA and KT） on the seedlings proliferation were studied by complete experiment and orthogonal design. The results showed that the optimum basic medium for G. sylvestre was N68;N68＋NAA 0. 05 mg/L＋2-ip 0.4 mg/L＋TDZ 0. 001 mg/L was the best for bud proliferation,and can be used in subculture;N68＋NAA 0.05 mg/L＋TDZ 0. 005 mg/L promoted rooting,can be used as rooting medium.

关 键 词：匙羹藤 组织培养 完全实验 正交设计 

分 类 号：Q943.1[生物学—植物学]