乌头碱类双酯型二萜生物碱水解反应的电喷雾质谱分析  

Analysis on Hydrolyzation of Aconitum Diester Diterpenoid Alkaloids by Electrospray Ionization Mass Spectrometry

作　　者：包懿[1,2] 宋凤瑞[1] 刘志强[1] 刘淑莹[1] 殷军[2] 

机构地区：[1]中国科学院长春应用化学研究所,绿色化学与过程实验室,吉林长春130022 [2]沈阳药科大学,中药学院生药实验室,辽宁沈阳110016

出　　处：《质谱学报》2009年第1期1-5,共5页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然基金(批准号:30672600,30472134)项目;973中医理论专项(2006CB504706)资助

摘　　要：阐明了乌头碱类双酯型二萜生物碱水解反应过程,确定水解反应的类型以及最佳水解条件。利用电喷雾离子阱质谱分析比较了生川乌提取液,制川乌提取液及其水解反应提取液中生物碱的差异。生川乌炮制后生物碱大多数由双酯型水解转化为单酯型,水解反应最容易发生的位置在C8位,水解反应的类型是烷氧键发生断裂的酯的碱性水解。The hydrolyzation process of aconitum diterpenoid alkaloids was studied, and the reaction type was defined according to its optimized experimental conditions. Electrospray ionization mass spectrometry （ESI-MS） was introduced to compare the difference in alka- loids types among the extraction of crude aconite roots and prepared aconite roots. After processing of crude aconite roots, most of the diester diterpenoid alkaloids converted to the monoester form, while C8 was the major location for such reaction. It is a typical alkali-catalyzed hydrolysis derived from the decomposition of the alkoxy chain.

关 键 词：乌头碱类二萜生物碱 双酯型二萜生物碱 电喷雾质谱 水解反应 

分 类 号：O657.63[理学—分析化学]