大鼠骨髓源性肝干细胞脾内移植后的分化  

Differentiation of Rat Bone Marrow Derived Hepatic Stem Cell after Transplantation into Spleen

作　　者：褚志予 刘晓明[1] 杨明智[2] 

机构地区：[1]青岛市传染病医院八病区,266033 [2]福建医科大学第一附属医院

出　　处：《青岛医药卫生》2009年第1期17-19,共3页Qingdao Medical Journal

摘　　要：目的探索大鼠骨髓源性肝干细胞(BDHSC)脾内移植后的分化情况。方法利用肝细胞生长因子(HGF)体外诱导大鼠BDHSC,并植入60只成年SD大鼠脾内,两组(n=30)分别于术后7天和14天处死动物后取脾,RT-PCR及免疫组化检测移植细胞的AFP及白蛋白的表达。结果移植后7天,AFPmRNA、白蛋白mRNA和AFP的阳性表达率分别为73.3%、46.7%、56.7%;移植后14天,AFPmRNA阳性表达率(23.3%)显著下降(P<0.01),白蛋白mRNA阳性表达率(20%)显著下降(P<0.05),AFP阳性表达率(26.7%)亦显著下降(P<0.05)。结论体外诱导大鼠BDHSC脾内移植后。Objective To explore the differentiation of rat bone marrow derived hepatic stem cell （BDHSC） after intrasplenical transplantation. Methods BDHSC was induced by hepatocytes growth factor in vitro, 7 days and 14 days after intrasplenical transplantation, 60 rats（two groups, n=30） were separately put to death for spleen, RT-PCR and immunohistochemistry were used to examine the expression of AFP and albumin in the BDHSC. Results 7 days after transplantation, the positive rate of AFP mRNA,albumin mRNA and AFP was 73.3% ,46.7% ,56.7%; 14 days after transplantation, the positive rate of AFP mRNA （23.3%） decreased significantly（P〈0.01）, the positive rate of albumin mRNA（20% ） decreased significantly（P〈0.05）, and the positive rate of AFP（26.7%） also decreased significantly（P〈0.05）. Conclusion BDHSCs in vitro might gradually lose their acquired characters of hepatocytes after intrasplenical transplantation.

关 键 词：骨髓源性肝干细胞 移植 分化 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]