蓝狐细小病毒的分离鉴定及其VP1基因序列分析  

Isolation and identification of blue fox parvovirus and sequence analysis of its VP1 gene

作　　者：刘海防[1] 胡传伟[2] 谢之景[1] 倪长鹏[2] 贾赟[2] 姜世金[1] 张兴晓[1] 杨笃宝[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]辽宁出入境检验检疫局技术中心,大连116001

出　　处：《兽类学报》2009年第1期81-85,共5页Acta Theriologica Sinica

基　　金：山东农业大学青年科技创新基金(23204);国家质检总局资助项目(2005IK047)

摘　　要：从泰安地区送检的疑是细小病毒感染的蓝狐粪便中分离到一株病毒。经理化特性鉴定、血凝谱鉴定、人工感染蓝狐等鉴定,表明所分离病毒为细小病毒。并且根据GenBank上发表的犬细小病毒(Canine parvovirus,CPV)、猫细小病毒(Feline parvovirus,FPV)核酸序列,设计扩增VP1基因的引物,采用PCR技术扩增所分离细小病毒的VP1全基因,将PCR产物克隆入pMD18-T载体,进行测序分析。结果,所分离细小病毒的VP1基因全长2256bp,编码727个氨基酸,与CPV和FPV参照株的VP1基因同源性在98.7%～99.5%。VP1基因的系统发生分析表明所分离病毒与FPV的亲源关系最为密切。所分离病毒VP1蛋白375位氨基酸残基与CPV的VP1蛋白氨基酸残基一致,但其223位、236位、246位、466位、707位、711位氨基酸残基与FPVVP1蛋白的氨基酸残基一致,该病毒VP1蛋白序列表现出了过渡型序列特征,介于FPV与CPV间的过渡类型,这说明所分离病毒为蓝狐细小病毒(Blue fox parvovirus,BFPV),命名为BFPV-TA,蓝狐可能在CPV的起源过程起到重要的作用。A parvovirus strain isolated from the feces of blue foxes in Tai＇ an, It was identified was parvovirus by a series of physicochemical assays, viral hemagglutination test and by examining animals exposed to it. The primers were designed based on the VP1 genes of the strains of CPV and FPV in Genbank. The VP1 gene of the isolate was amplified by PCR, which was cloned into pMD18 - T vector, sequenced and analyzed. The ORF of the VP1 gene of the isolate included 2 256 bp and encoded 727 amino acids. The nucleotide homologies of VP1 genes between the isolate and the CPV, FPV references are from 98. 7% to 99. 5%. Phylogenetic analysis showed that the isolate and FPV had the smaller inheritance distances. The 375th amino acids of the VPlprotein of the isolate were identical to those of CPV, but the 223th, 236th, 246th, 466th, 707th and 71 lth amino acids of the VPI protein were consistent with those of FPV. The sequence of the VP1 gene of the isolate suggested transition between FPV and CPV. It implied that the isolate was blue fox parvovirus, named blue fox parvovirus Taian isolate （BFPV -TA ） , and blue fox played an important role in CPV appearance.

关 键 词：蓝狐细小病毒 VP1基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]