胆囊癌及其良性病变组织中CCL3及其受体表达的临床病理意义  

Expression of CCL3 and its receptor CCR1 in gallbladder adenocarcinoma and its benign lesions and its clinico- pathological significance

作　　者：杨竹林[1] 兰思根[1] 付汐[1] 苗雄鹰[1] 

机构地区：[1]中南大学湘雅二医院肝胆疾病研究室,长沙410011

出　　处：《中华肝胆外科杂志》2009年第1期25-27,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的研究胆囊癌、慢性胆囊炎和胆囊结石组织中CCL3及其受体CCR1表达水平及其临床病理意义。方法108例胆囊腺癌、15例慢性胆囊炎和20例胆囊结石组织标本常规制作石蜡包埋切片，CCL和CCR1染色方法为SP免疫组化法。结果胆囊腺癌CCL3和CCR1表达阳性率及其评分明显高于慢性胆囊炎和胆囊结石组织（P〈0．01）；腺瘤癌变或高分化腺癌、肿块最大径〈2cm、淋巴结未转移及未侵犯周围组织病例CCL3和CCR1的表达阳性率及其评分明显低于低分化腺癌、肿块最大径≥2cm、淋巴结转移和侵犯周围组织病例（P〈0．05或P〈0．01）；胆囊癌中CCL3和CCR1表达呈高度一致性（P〈0．01），其评分值呈高度密切正相关（r=0．68，P〈0．01）。结论CCL3及其受体CCR1表达可能是反映胆囊癌发生、进展、生物学行为和预后的重要生物学标记物。Objective To study the expression of CCL3 and its receptor CCR1 in gallbladder adenocarcinoma and gallstone tissues and explore its clinicopathological significance. Methods SP im munohistochemical method was used to detect expression of CCL3 and CCR1 in routinely paraffin embedded sections from 108 cases of gallbladder adenocarcinoma, 1,5 of chronic cholecystitis and 20 of gallstone. Results The positive rates and scores of CCL3 and CCR1 were significantly higher in gallbladder adenocarcinoma than in chronic cholecystitis and gallstones （P〈0.01）. Meanwhile,they were markedly lower in the cases of adenomatous canceration or highly differentiated adenocarcinoma, the maximal diameter of mass 〈2 cm, without lymph node metastasis or without infiltration of regional tissues than in those of lowly differentiated adenocarcinoma, maximal diameter of mass ≥2 cm, with lymph node metastasis or with infiltration of regional tissue （P〈0. 05 or 0. 01）. Conclusion The CCL3 and its receptor CCR1 might be important biological markers for reflecting the carcinogenesis, progression,biological behaviors and prognosis of gallbladder adenocarcinoma.

关 键 词：胆囊肿瘤 化学趋化因子类 C-C化学趋化因子受体-1 免疫组织化学 

分 类 号：R686[医药卫生—骨科学]