蜱中玫瑰单胞菌菌株的分离与鉴定  

Isolation and identification of Roseomonas strain from the ticks

作　　者：邱尔臣[1] 张芳[1] 刘玮[1] 吴晓明[1] 杨红[1] 曹务春[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《中华微生物学和免疫学杂志》2009年第1期29-32,共4页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：国家自然科学基金资助项目（30600506）

摘　　要：目的确定从蜱中分离的、使用常规方法不能鉴定的3株粉红色细菌的分类学位置。方法使用16SrDNA序列分析和同源性检索方法及细菌的形态学、生化特性及脂肪酸分析等鉴定方法。结果3株细菌为粉红色的革兰阴性需氧小球杆菌，最适生长温度为37℃，在含有5％以上NaCl的培养基中不能生长。该菌能发酵葡萄糖、利用棕檬酸盐，接触酶试验阳性、脲酶试验阳性、紫外（UV）吸收试验阳性。主要脂肪酸成分为C16：0、C17：0、C18：1 ω9c和C19：0cyc。16SrDNA序列分析和同源性检索发现3株细菌与颈玫瑰单胞菌（Roseomonas cervicalis）同源性最高，达99．1％。结论这3株细菌在分类学上属于玫瑰单胞菌（Roseomonas），但是和已经报道的种都不相同，可能是一个变种。蜱可能是其潜在的媒介宿主。Objective To identify the three strains of pink-pigmented bacteria isolated from ticks. Methods 16S rDNA sequence analysis, 16S rDNA similarity searching, bacterial morphological, physio- logical and biochemical tests and fatty acids analysis were used. Results The bacterial strains were pinkpigment, gram negative, strictly aerobic coccobacillus. They grow optimally at 37℃, while not in the pres- ence of 〉15% NaCl. The tests showed positive result for urease, D-glucose, citrate, UV absorption, and catalase. The predominant fatty acids were C16：0、C17：0、C18：1 ω9c and C19：0 cyc. Comparative 16S rDNA sequences analysis revealed that the strains were closely related to Roseomortas cervicalis and could reach up to 99.1% identity. Conclusion Based on phenotypic, biochemical and phylogenetic data, we propose these three isolates represent distinct species in genus Roseomonas, of which ticks may serve as a potential vector.

关 键 词：玫瑰单胞菌 草原革蜱 分离 鉴定 

分 类 号：R446.5[医药卫生—诊断学]