肉毒毒素A对大鼠皮肤及创面组织中SP、CGRP、TGF—β1和α-SMA的影响  

Effect of botulinum toxin type A on the expression of substance P, calcitionin gene-related peptide, transforming growth factor beta-1 and alpha smooth muscle actin A in wound healing in rats

作　　者：王琳[1] 邰宁正[1] 范志宏[1] 

机构地区：[1]上海交通大学医学部附属仁济医院整形外科,200127

出　　处：《中华整形外科杂志》2009年第1期50-53,共4页Chinese Journal of Plastic Surgery

基　　金：上海市科学技术委员会资助项目（07JC14038）

摘　　要：目的探讨肉毒毒素A对周围神经末梢释放的感觉神经肽P物质、降钙素基因相关肽及创面组织表达TGF-β1和α-平滑肌肌动蛋白的影响。方法60只SD大鼠随机分为空白对照组（C组）、低剂量组（L组）和高剂量组（H组），每组20只大鼠。肉毒毒素A预注射后7d，在鼠背部建立以4个注射点为中心的创面模型，创面面积1cm×1cm。于术前及术后3、7、14d，测量创面面积，免疫组织化学和Axioplan 2 Imaging图像分析软件检测多聚甲醛灌注后创面P物质、降钙素基因相关肽以及创面愈合中的重要分子TGF-β1和α-平滑肌肌动蛋白的积分吸光度A值，分析肉毒毒素A对上述物质表达的影响及意义。结果①各组创面于术后14d基本愈合。术后3、7d的3组创面面积组间比较差异无统计学意义（P〉0．05）；②3组各时相点的皮肤及创面组织中P物质、降钙素基因相关肽、TGF-β1和α-平滑肌肌动蛋白的表达，组间比较差异有统计学意义（P〈0．01），且H组〈L组〈C组。结论①局部皮下注射肉毒毒素A不影响创面愈合的时间和速度；②肉毒毒素A可减少创面愈合过程中周围神经末梢对P物质、降钙素基因相关肽的释放和创面愈合过程中TGF-β1和α-平滑肌肌动蛋白的表达，且作用呈剂量依赖性。Objective To investigate the effect of botulinum toxin type A on the expression of substance P（SP）, calcitionin gene-related peptide （ CGRP）, transforming growth factor beta- 1 （ TGF-β1） and alpha smooth muscle actin A（α-SMA） in wound healing. Methods 60 rats were randomly divided into group C （control）. group L （low-dose） and group H（high-dose）, with 20 rats in each group. The wound-healing model was established by excision of four full-thickness skin（ 1 cm × 1 cm, around the injection site） on the back of all SD rats on the 7th day after BTA injection. The wound size was measured and the expression of SP, CGRP, TGF-β1, and α-SMA in wound granulation tissue was assayed by immunohistochemical staining and computerized image analysis before operation, and 3 days, 7 days and 14 days after operation. Results All the wounds healed 14 days after operation. The wound size in L and H group was not significantly different with that in C group on the 3rd day and 7th day after operation. The positive immuno-staining of SP, CGRP, TGF-β1 and α-SMA in group L and H was significantly weaker than those in C group. Meanwhile, the positive immuno-staining of all above substances in H group was weaker than those in L group significantly. Conclusions Botulinum toxin type A can decrease the expression of SP, CGRP, TGF-β1 and α-SMA in wound healing in a dose-dependent manner with no effect on the healing time.

关 键 词：肉毒杆菌毒素 A型 伤口愈合 瘢痕 

分 类 号：R641[医药卫生—外科学]