砷中毒对小鼠脏器脂质过氧化的影响及VE干预作用的研究  

Experimental study of arsenic and the antagonistic action of vitamin E on lipid peroxidation in the organs of mice

作　　者：王茜[1] 李玉华[1] 李树林[2] 田强[1] 贾汉[1] 

机构地区：[1]新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室,乌鲁木齐830054 [2]新疆乌鲁木齐市疾病预防控制中心

出　　处：《中国地方病防治》2009年第1期9-12,共4页Chinese Journal of Control of Endemic Diseases

摘　　要：目的研究不同水平的砷对小鼠脏器组织脂质过氧化的影响,并观察维生素E(VE)对砷毒作用的干预作用。方法昆明种小鼠按2因素3水平析因实验设计分为9组,不同实验组的动物通过喂饲法给三氧化二砷和VE复制亚慢性砷中毒动物模型。三氧化二砷的分组剂量按照其对小鼠经口半数致死量LD50进行分组,VE按照我国青少年每日推荐量进行分组。2个月后,采用试剂盒,研究砷对小鼠脏器组织中超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量的影响以及给于VE处理后的干预作用。结果染毒组动物脏器组织中SOD、GSH-Px活力低于正常对照组动物(P<0.05),MDA含量高于正常对照组(P<0.05);VE处理后动物脏器组织中SOD、GSH-Px活力增强,MDA含量降低(P<0.05)。结论砷能引起小鼠脏器组织中氧化与抗氧化系统失衡,VE可拮抗砷对小鼠脏器氧化水平与抗氧化能力的影响。Objective To study the influence of arsenic and the antagonistic action of vitamin E on lipid peroxidation in the organs of mice. Methods Kunming mice were divided into nine groups according to the factorial experiment design of two factors and three levels. The mice of different groups were fed with both various amounts of arsenic in drinking water and vitamin E for two months to produce the animal model of subchronic arsenic poisoning. The mice were fed the different dose of arsenic the LDs0 and the dose of vitamin E according to the youngsters RDA （ recommended dietary allowance）. The activity of superoxide dismutase （SOD） , glutathione peroxidase （GSH -Px） and the contents of malondiasldehyde （MDA） in the organs of mice were measured by test kit. Results The SOD and CSH -Px contents were lower（ P 〈 0.05） , while the MDA contents were higher （ P 〈0.05） in arsenic groups. Correspondingly, the SOD and GSH -Px contents were higher （ P 〈0.05）, while the MDA contents were lower （ P 〈 0.05 ） in the groups added with various dose of vitamin E （ abbreviated by Vit E ＋ As）. Conclusions Lipid peroxidation （LPO） and the anti - lipid peroxidation might play a vital role in the mechanism of arsenism. There was an interaction between arsenic and vitamin E. Vitamin E has an antagonistic action against the arsenism through mataining the balance of the lipid peroxidation and anti -lipid peroxidation system.

关 键 词：砷 脂质过氧化 维生素E 

分 类 号：R599.9[医药卫生—内科学]