冻干甲型肝炎减毒活疫苗诱导的人体特异性免疫应答  

Specific Immune Responses Induced by Freeze-dried Live Attenuated Hepatitis A Vaccine in Humans

作　　者：忻亚娟[1] 庄昉成[1] 姜立民[1] 钱汶[1] 唐彩华[1] 陈悦青[1] 吴洁[1] 柴少爱[1] 

机构地区：[1]浙江省医学科学院病毒病研究所,杭州310013

出　　处：《中国生物制品学杂志》2009年第2期180-182,共3页Chinese Journal of Biologicals

摘　　要：目的观察人体接种冻干甲型肝炎减毒活疫苗(H2株)后产生的特异性免疫应答。方法选择16名血清甲型肝炎抗体阴性的健康志愿者,接种1针冻干甲型肝炎减毒活疫苗,接种前和接种后2、4、8、12、156周(3年)采血,采用ELISA检测血清抗HAVIgG抗体;采用流式细胞术检测全血各淋巴细胞亚群CD3+、CD4+、CD8+的百分率及表达细胞因子IFN-γ和IL-4的阳性细胞百分率;采用ELISPOT法检测外周血淋巴细胞分泌IFN-γ的斑点形成细胞(SFC)。结果接种疫苗后2周,表达IL-4的阳性细胞百分率与接种前相比显著升高;接种后4周,CD4+淋巴细胞亚群百分率与接种前相比显著升高;接种后8周,抗体100%阳转;接种后3年,抗体和分泌IFN-γ的SFC有一项以上阳性者占85.7%(12/14)。结论接种冻干甲型肝炎减毒活疫苗,能诱导机体产生良好的体液免疫和细胞免疫应答。Objective To observe the specific immune responses induced by freeze-dried live attenuated hepatitis A （HA） vaccine（H2 strain）in humans. Methods Sixteen healthy volunteers negative for serum HA antibody were inoculated with one dose of freeze-dried live attenuated HA vaccine. The blood samples were collected before and 2, 4, 8, 12 and 156 weeks （3 years） after inoculation and determined for anti-HAV IgG by ELISA, for the percentages of CD3^＋, CD4^＋ and CD8^＋ lymphocyte subsets as well as positive cells expressing IFN-γand IL-4 by flow cytometry, and for spot forming cells （SFCs） secreting IFN-γ by ELISPOT method. Results The percentage of positive cells expressing IL-4 2 weeks after inoculation was significantly higher than that before inoculation. The percentage of CD4^＋ lymphocyte subsets 4 weeks after inoculation was significantly higher than that before inoculation. Eight weeks after inoculation, the anti-HAV IgG positive rate reached 100%. Three years after inoculation, 85. 7% （12/14） of volunteers were positive for anti-HAV IgG and/or SFCs. Conclusion Freeze-dried live attenuated HA vaccine has induced good humoral and cellular immune responses in humans.

关 键 词：冻干甲型肝炎减毒活疫苗(H2株) 体液免疫 细胞免疫 

分 类 号：R373.21[医药卫生—病原生物学] R392-33[医药卫生—基础医学]