人工合成启动子SAR在拟南芥中的表达分析  

Study on Expression Pattern of the Synthetic Promoters SAR in Arabidopsis

作　　者：张秀春[1] 崔百明[1,2] 任艳利[1,3] 李文彬[1] 彭明[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术研究所,海口571101 [2]石河子大学生物科学学院新疆兵团绿洲生态农业重点实验室,新疆石河子832003 [3]伊犁师范学院生物化学与环境科学系,新疆伊宁835000

出　　处：《热带亚热带植物学报》2009年第1期49-53,共5页Journal of Tropical and Subtropical Botany

基　　金：国家重点基础研究发展规划(973计划)项目(2004CB117307);中国热带农业科学院中央级公益性科研院所基本科研业务费专项基金(热带生物技术研究所项目、项目编号ITBBZ×2008-1-1)资助

摘　　要：利用植物防御基因中的病原诱导响应元件和最小35S启动子(-62～+1),人工合成了启动子SAR,并以GUS基因为报告基因,在转基因拟南芥中分析了合成启动子的表达特性。通过对转基因拟南芥GUS组织染色的分析表明:SAR启动子在子叶、毛刺、根茎交接处和根系中优势表达,在老叶中的表达量高于幼叶,说明SAR启动子具有组织和发育表达特异性。The promoter SAR by combining mini-35S promoter （-62 to ＋ 1） and several pathogen responsive c/s-acting elements derived from defense genes in plant was synthesized, and the expression pattern of these synthetic promoters was studied by using report gene GUS （[^-glucuronidase） transformed into Arabidopsis. The results showed that synthetic promoter SAR could make GUS predominant expression in cotyledon, trichome, rhizome conjoit region, root system and old leaves, and the expression in old leaves was higher than that in young leaves. It suggested that SAR promoter had tissue and development specific expression.

关 键 词：合成启动子SAR GUS基因 拟南芥 基因表达 

分 类 号：Q943.2[生物学—植物学]