丹参多酚酸盐对猪急性心肌梗死后心肌细胞凋亡和心功能的影响  

Effects of salvianolate on cardiomyocytic apoptosis and heart function in a swine model of acute myocardial infarction

作　　者：王明伟[1] 张殿福[1] 唐建金[1] 陈波[1] 王连生[1] 杨志健[1] 黄峻[1] 

机构地区：[1]南京医科大学第一附属医院心内科,江苏南京210029

出　　处：《中西医结合学报》2009年第2期140-144,共5页Journal of Chinese Integrative Medicine

基　　金：上海绿谷制药有限公司科研基金资助项目;江苏省自然科学基金资助项目(No.BK2007254);人事部留学人员科研择优课题资助项目(No.DG216D5021)

摘　　要：目的:观察丹参多酚酸盐对猪急性心肌梗死后心肌细胞凋亡及心功能的影响。方法:苏中幼猪21只,随机分为模型组和低、高剂量丹参多酚酸盐组。3组均经开胸结扎冠状动脉左前降支制作心肌梗死模型,造模成功后当日,高剂量丹参多酚酸盐组给予400mg丹参多酚酸盐加250mL5%葡萄糖生理盐水静脉滴注;低剂量丹参多酚酸盐组给予200mg丹参多酚酸盐加250mL5%葡萄糖生理盐水静脉滴注;模型组给予等体积的5%葡萄糖生理盐水静脉滴注。1次/d,连续给药7d。造模后4周,行核素门控心肌灌注显像评价各组动物心功能;处死动物,取心脏标本做石蜡切片,原位缺口末端标记法染色,高倍镜下计数心肌细胞凋亡指数。结果:高、低剂量丹参多酚酸盐组动物梗死周边缺血区的心肌细胞凋亡指数均明显下降,高剂量丹参多酚酸盐组与模型组比较,差异有统计学意义(P<0.05)。心肌梗死后4周行核素门控心肌灌注显像,3组动物的梗死区均可见放射性稀疏区,但高、低剂量丹参多酚酸盐组放射性稀疏区较模型组明减显小,高剂量丹参多酚酸盐组心肌灌注得分与模型组相比,差异有统计学意义(P<0.05);高剂量丹参多酚酸盐组动物的左室射血分数也高于模型组(P<0.05)。结论:静脉维持应用丹参多酚酸盐,能够减少猪急性心肌梗死后心肌细胞凋亡,促进梗死心脏心功能的改善,发挥心脏保护作用。Objective： To observe the effects of salvianolate on cardiomyocytic apoptosis and heart function in a swine model of acute myocardial infarction （AMI）. Methods： A total of 21 young swines were randomly divided into untreated group, low-dose salvianolate （LS） group and high-dose salvianolate （HS） group （7 in each group）. AMI was induced by ligaturing left anterior descending coronary artery. After the operation, 400 or 200 mg salvianolate dissolved in 250 mL 5% glucose saline was administered by intravenous drip to swines in the HS group and the LS group respectively for 7 days. The swines in the untreated group were administered with 250 mL 5% glucose saline. The left ventricular ejection fraction （LVEF） and myocardial perfusion were measured by gated myocardial perfusion imaging at the end of the 4th week after operation. And myocardial apoptosis was detected by TdT-mediated dUTP-biotin nick end labeling （TUNEL） method. Apoptosis index was calculated under an optical microscope. Results. Myocardial apoptotic index in the edge of myocardial infarction was decreased in the HS group and the LSgroup, which in the HS group was lower than that in the untreated group （P〈0.05）. Radioactive defect regions were found by gated myocardial perfusion imaging in all the three groups, but those in the HS group and the LS group were less than those in the untreated group （P〈0.05）, And the levels of myocardial perfusion and LVEF in the HS group were significantly higher than those in the untreated group （P〈0.05）. Conclusion： Salvianolate administered by intravenous drip can inhibit the cardiomyocytic apoptosis and improve the function of heart after AMI in swines.

关 键 词：心肌梗死 丹参多酚酸盐 细胞凋亡 猪 

分 类 号：R285.5[医药卫生—中药学]