中药熏蒸治疗大鼠佐剂性关节炎的疗效及抗炎机制  

Treatment of adjuvant arthritis in rats with Chinese herbal fumigation:efficacy and mechanism

作　　者：沈鹰[1] 陆继娣[2] 

机构地区：[1]广州军区广州总医院中医科,广东广州510010 [2]安徽省六安市人民医院风湿科,安徽六安237005

出　　处：《中西医结合学报》2009年第2期157-162,共6页Journal of Chinese Integrative Medicine

基　　金：广东省中医药管理局项目(No.1050054)

摘　　要：目的:观察中药熏Ⅰ方熏蒸对大鼠佐剂性关节炎(adjuvant arthritis,AA)的疗效,并探讨其抗炎机制。方法:雄性Wistar大鼠50只,随机分为正常组、模型组、蒸馏水熏蒸组、低浓度中药熏蒸组和高浓度中药熏蒸组,每组10只。除正常组外,用弗氏完全佐剂皮下注射建立AA模型。造模成功后,分别采用低、高浓度中药熏Ⅰ方和蒸馏水进行熏蒸,观察中药熏蒸对足肿胀度,踝关节病理,踝关节炎性细胞因子白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)表达及血清中该3种炎性细胞因子含量的影响。结果:治疗后与模型组比较,两种浓度中药熏蒸组大鼠足肿胀程度明显减轻(P<0.05),踝关节炎性细胞浸润、纤维组织增生和滑膜细胞增生等病理改变明显好转(P<0.05,P<0.01),踝关节IL-1β、TNF-α、ICAM-1的阳性表达显著下调(P<0.05),血清IL-1β、TNF-α和ICAM-1含量显著降低(P<0.05)。各熏蒸组之间比较,高浓度中药熏蒸组效果明显优于低浓度中药熏蒸组和蒸馏水熏蒸组(P<0.05,P<0.01)。结论:中药熏Ⅰ方熏蒸可有效治疗大鼠AA,其作用机制可能与抑制炎性细胞因子IL-1β、TNF-α、ICAM-1的表达,从而抑制或减轻病变关节的炎症反应有关。Objective： To observe the efficacy of fumigation with Fumigant Ⅰ formula, a compound traditional Chinese herbal medicine, in treating adjuvant arthritis （AA） in rats and to explore its anti-inflammation mechanism. Methods： Fifty male Wistar rats were randomly divided into normal control group, untreated group, distilled-water fumigation group, and low- and high-dose herb fumigation groups. Except for the normal control group, the other rats were used to induce AA by complete Freund＇s adjuvant injection. After treatments, the degree of foot swelling, the pathological changes and expressions of the inflammatory cytokines including interleukin- 1β （IL-1β）, tumor necrosis factor-α （TNF-α） and intercellular adhesion molecule-1 （ICAM-1） in the ankle joint were detected. The contents of these cytokines in the serum were also observed. Results： In two Chinese herbal fumigation groups, as compared with the untreated group, foot swelling was obviously reduced （P〈0. 05）, the pathological changes of the ankle joint, such as inflammatory cell infiltration, fibrous tissue and synovial cell proliferation in the ankle joint were obviously improved （P〈0. 05,P〈0.01）, the positive expressions of IL-1β, TNF-α, ICAM-1 in the ankle joint were significantly down-regulated （P〈0.05）; and the contents of these inflammatory cytokines in the serum were obviously decreased （P〈0. 05）. The treatment effect in high-dose herb fumigation group was better than that in lowdose herb fumigation group and that in distilled-water fumigation group （P〈0.05, P〈0.01）. Conclusion： Fumigation with Chinese herbal medicine has a positive therapeutic effect on AA rats, and its anti-inflammatory mechanism may be related with inhibiting inflammatory cytokines IL-1β, TNF-α and ICAM-1.

关 键 词：中药熏蒸 佐剂性关节炎 白细胞介素-1Β 肿瘤坏死因子-α 细胞间黏附分子-1 大鼠 

分 类 号：R244.9[医药卫生—针灸推拿学]