绵羊痘病毒甘肃流行株膜蛋白ORF23基因的克隆及序列分析  

Cloning and analysis of membrane protein encoding ORF23 from sheeppox virus Gansu strain

作　　者：张冬峰[1] 郑亚东[1] 骆学农[1] 王晓霞[1] 陈轶霞[2] 李辉[1] 侯俊玲[1] 景志忠[1] 才学鹏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室／农业部兽医公共卫生重点实验室／中国农业科学院草食动物疫病重点实验室,甘肃兰州730046 [2]西北民族大学生命科学与工程学院,甘肃兰州730046

出　　处：《福建农林大学学报（自然科学版）》2009年第1期44-48,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：中国农业科学院兰州兽医研究所所长基金项目(06-11);兰州市城关区农业科技攻关项目(07-32)

摘　　要：从甘肃景泰县疑似患羊痘的绵羊皮肤组织中提取基因组DNA,PCR扩增膜蛋白ORF23基因.序列分析表明,该基因由1113个核苷酸组成,编码370个氨基酸,A+T含量占69.90%,G+C含量仅占30.10%.绵羊痘病毒甘肃株ORF23基因与A株、NISKHI株之间的核苷酸同源性分别为99.6%和99.5%,氨基酸同源性分别为98.7%和98.4%;与皮肤疙瘩病病毒NW-LW株和山羊痘病毒Pellor株之间的核苷酸同源性均为98.2%.结构预测显示,ORF23膜蛋白为非分泌蛋白,具有一个O连结糖基化位点.可见,ORF23膜蛋白在不同的绵羊痘病毒流行株之间非常保守,具有潜在的疫苗和诊断研究价值.Genomic DNA was extracted from the skin tissue of suspected capripox sheep in Jingtai, Gansu Province, and the mem- brane-protein encoding ORF23 gent was cloned by PCR and sequenced. The results showed that the length of open reading frame of membrane protein encoding ORF23 gene was 1113 bp, coding a 370-amino acid protein. Its nucleotide component was imbalance with 69.90% of A ＋ T content and 30.10% of G ＋ C. The homology of nucleotide and deduced amino acid sequence between sheep- pox virus Gansu strain, sheeppox virus A train and NISKHI strain was 99.6% , 99.5% and 98.7% , 98.4% reapectively. ORF23 gene of the sheeppox virus Gansu strain shared 96.5% and 94.8% identity with that of Lumpy skin disease virus Neethling Warmbaths LW strain and goatpox virus Pellor strain. Structural analysis of the deduced ORF23 protein indicated that the ORF23 antigen was not a secretory protein with one O-linked glycosylation sites and one phospholipase domain. It suggested that the ORF23 mem- brane protein is conserved among sheeppox field strains and has potential value in development of vaccine or/and diagnosis.

关 键 词：绵羊痘病毒 ORF23 膜蛋白 

分 类 号：S852.654[农业科学—基础兽医学]