中药续断含药血清对成骨细胞增殖和骨基质蛋白产生的影响  

Effects on rats serum containing Radix Dipsaci on the proliferation,AKP activity and OC secretion of osteoblasts in vitro

作　　者：王威[1] 史红[1] 何永志[1] 顾志敏[1] 郑纺[1] 刘友刚[1] 王超[1] 胡利民[1] 

机构地区：[1]天津中医药大学

出　　处：《中国骨质疏松杂志》2009年第2期103-106,共4页Chinese Journal of Osteoporosis

基　　金：国家科技部支撑计划名优中成药大品种二次开发关键技术的研究项目资助(2007BAI47B04)

摘　　要：目的观察中药续断含药血清对体外培养的成骨细胞增殖和骨基质蛋白产生的影响。方法①制备大鼠含药血清：取雌性与雄性Wistar大鼠各12只，按性别随机分为4组，分别为：雄性给药组，雄性对照组，雌性给药组，雌性对照组，每组各6只，给药组按体重（4．5g生药／kg体重）分别给予续断水提液灌胃、对照组按体重分别给予等量生理盐水，灌胃1W后取血，离心提取血清。②分离、培养大鼠的原代成骨细胞。③将各组大鼠血清稀释为2．5％、5％和10％3个浓度分别培养大鼠成骨样细胞，采用四氮唑蓝法（MTT）检测细胞增殖能力，同时采用生化和放免法进行了细胞碱性磷酸酶（AKP）检测和细胞培养液骨钙素（OC）检测。结果当培养基中含药血清浓度为2．5％、5％时，雌性给药组成骨细胞增殖率、AKP和OC的量均高于雄性给药组和雌性对照组（P〈0．01）；当血清浓度为10％时，雌性给药组与雌性对照组比较，存在显著性差异（P〈0．05）。雌性给药组与雄性给药组比较，无显著性差异（P〉0．05）。在各浓度的血清中，雄性给药组与雄性对照组之间均无显著性差异。结论中药续断的含药血清具有刺激骨基质蛋白（碱性磷酸酶和骨钙素）生成和分泌的作用，并具有刺激成骨细胞增殖的作用。这种作用在雌性大鼠的血清中表达强于雄性大鼠。Objective To observe the effect of rats serum containing Radix Dipsaoi at different concentration and different sex on cell proliferation and AKP activity and OC secretion of osteoblasts in vitro. Methods ①Preparation of the rat scrum containing drug： Wistar rats 24, half in male and half in female, were randomly divided into four groups（male control group（ Ⅰ ）, male drug-added group（ Ⅱ ）, female control group（ Ⅲ ）, female drug-added group（ Ⅳ ）. After being treated with Radix Dipsaci （4.5 g crude drug/kg weight）, and saline, respectively with gastric perfusion according to body mass for one week, blood was gained. After centrifuging operation, supernatant fluid was obtained. ②osteoblasts were isolated and cultured. O Quantitative analysis on proliferation of osteoblasts： The ceils were disposed with different concentration of rat serum, and divided into 2.5% ,5% and 10% Radix Dipsaci group. The proliferation, AKP activity and OC secretion of cells was tested. Results ①Compared with the male drug-added group（0.3613 ± 0.0983）, female drug-added group（0.5690 ± 0.0295） could increase absorbance value and proliferation of osteoblasts, and there were significant difference（ P 〈 0.05） when the medium with 2.5% concentration of Radix Dipsaci rat serum. There were also significant difference（ P 〈 0.01）between female drug-added group and female antitheses group（0.3787 ± 0.0476）. ②when the medium was 5 % concentration of Radix Dipsaci rat serum, Compared with the male drug-added group（0.3560 ± 0.0482）, female drug-added group （0.8793 ± 0.1007） could increase absorbance value and proliferation of osteoblasts, and there were Significant difference （P 〈 0.01 ）. There were Significant difference （P 〈 0.01 ） between female drug-added group and female antitheses group（0.3863 ± 0.0180）, too. ③When the medium was 10% concentration of Radix Dipsaci rat serum, there was significant difference （P 〈 0.05）between female drugadded grou

关 键 词：成骨细胞 含药血清 续断 AKP活性 OC分泌 

分 类 号：R285.5[医药卫生—中药学]