金雀异黄酮对去卵巢大鼠椎体力学性能的影响  

Effects of genistein on the mechanical properties of vertebrae in ovariectomized rats

作　　者：马育林[1] 戴如春[2] 盛志峰[2] 金艳[2] 张玉海[3] 廖二元[2] 

机构地区：[1]南方医科大学附属小榄人民医院,中山528415 [2]中南大学湘雅二医院 [3]海南省人民医院

出　　处：《中国骨质疏松杂志》2009年第2期126-130,110,共6页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目(30400514);湖南省自然科学基金(06JJ50029);卫生部临床学科重点建设资助项目(2004-468-50)

摘　　要：目的探讨皮下注射金雀异黄酮对OVX大鼠椎体力学性能的影响及骨力学性能的决定因素。方法40只7月龄雌性sD大鼠随机分为去卵巢组（OVX组）、假手术组（SHAM）、OVX＋1713雌二醇干预组（EST，10硝（kg·d））、OVX＋金雀异黄酮干预组（GEN，5mg／（kg·d））。手术后15w第5腰椎进行压缩试验，第6腰椎先行显微CT扫描测量骨密度和微结构参数，然后进行疲劳损伤试验，最后行大块组织品红染色、塑料包埋和磨片，磨片用于微损伤、骨细胞密度检测。结果去卵巢后15w，与SHAM组相比，OVX大鼠椎体骨密度、微结构参数、骨细胞密度和最大应力均降低（P〈0．05），微破裂密度和面密度增加（P〈0．05）。GEN组骨小梁连接密度较OVX组增加（P〈0．05），其他微结构参数与OVX组差异无统计学意义。与OVX组相比，GEN和EST替代组骨细胞密度和最大应力均增加（P〈0．05），微破裂密度和面密度减少（P〈0．05）。OVX组、GEN和EST替代组之间骨密度和弹性模量差异无统计学意义。结论金雀异黄酮对去卵巢大鼠椎体骨力学性能的维持不依赖BMD和微结构变化。Objective To investigate the effects of subcutaneous injection of genistein on the biomechanical properties of vertebrae in ovariectomized rats and the determinants of bone strength. Methods 40 Spragne-Dawley （SD） rats,7 months old, were randomly divided into 4 groups, ovariectomized （OVX）, shamoperated （sham）, OVX and treated with 17β-estradiol （EST, 10 μg/（kg^-1·day^-1 ）） or genistein （GEN, 5 mg/（kg^-1 ·day^-1 ））. At 15 weeks postovariectomy, the compressive test was performed on the I5 vertebral body. Micro-computed tomography （μ-CT） was used to measure microstructure parameters and bone mineral density （BMD） of L6 vertebral body. After fatigure damage testing, L6 vertebral body was bulk-stained in 1% basic fuchsin and embedded in methylmethacrylate. Mounted bone slices were used to measure microcrack parameters and osteocyte density（ Or. D/T. Ar）. Results At 15 weeks postovariectomy, maximum loading （ML） and Or. N/T. Ar were significantly lower in OVX rats than other groups. ML decreased significantly in GEN group than in SHAM group. Microcrack density （ Cr. Dn）, microcrack surface density （ Cr. S. Dn） and microcrack length （ Cr. Le） increased significantly in OVX group than in other groups. Volumetric BMD （vBMD） was significantly lower in OVX, EST and GEN group than in SHAM group. Bone mineral content （BMC） was significantly lower in OVX group than in SHAM group. Bone volume fraction （BV/TV） and trabecular number （Tb. N） significantly decreased in OVX, EST and GEN groups than in SHAM group. Structure model index （SMI） was significantly lower in SHAM group than in OVX,EST and GEN groups. Trabecular separation （Tb. Sp） was increased significantly in OVX group than in SHAM and EST groups. There were no significantly differences of trabecular thickness （Tb, Th） between SHAM, GEN and OVX groups, but To, Th was significantly lower in EST group than in SHAM group. Connectivity density （Corm. D） was significantly lower in OV

关 键 词：金雀异黄酮 生物力学 骨密度 微结构 骨细胞密 

分 类 号：R285.5[医药卫生—中药学]