两种不同降钙素对体外培养破骨细胞影响的实验研究  

The effects of two kinds of calcitonin on osteoclasts in vitro

作　　者：崔维顶[1] 李翔[1] 刘志祥[1] 

机构地区：[1]南京医科大学第一附属医院骨科

出　　处：《中国骨质疏松杂志》2009年第2期131-134,共4页Chinese Journal of Osteoporosis

摘　　要：目的观察不同浓度鳗鱼降钙素与鲑鱼降钙素对体外分离培养的破骨细胞数量和活力的影响。方法从新生幼兔四肢长骨中机械分离出破骨细胞，分别接种于盖玻片及骨薄片上，加入两种不同种类的降钙素，抗酒石酸酸性磷酸酶（TRAP）染色观察盖玻片上破骨细胞的数量和形态，JD801图像分析软件分析骨片上骨吸收陷窝的面积。结果两种降钙素的各个浓度组（分别是10^-12mol，L、10^-10mol／L、10^-8mol／L）均对贴壁生长的破骨细胞数量有明显抑制作用，并呈剂量相关性。骨吸收陷窝面积分析结果显示，两类降钙素均对破骨细胞吸收功能有明显抑制作用，呈剂量相关性。无论是盖玻片培养还是骨薄片培养，益钙宁和密盖息各相同浓度组间两两对照比较差异均无显著性（P〉0．05）。结论两种不同种类降钙素对体外培养的破骨细胞数量和骨吸收活性的抑制能力相仿。Objective To observe the effects of the different concentrations of eel calcitonin （eCT） and salmon calcitonin （sCT） on proliferation and activation of osteoclasts in vitro. Methods The osteoclasts isolated from the long bones of neonatal New Zealand＇s rabbits were cocultured in bone slices and cover slips with different concentrations of eCT and sCT in M199. Numbers of the osteoclasts were calculated and the conformation of osteoclasts was observed after being stained with tartrate-resistance acid phosphatase （TRAP）. The resorptive areas on slices stained by toluding blue were counted bye Image-JD801 software. Results The number of osteoclasts decreased with the increase of eCT and sCT concentrations after incubation in cover slips for 2 days（ 10^-12 mol/L, 10^-10 mol/L and 10^-8 mol/L）（ P 〈 0.01 ）. The resorptive areas were decreased in a dose-dependent manner with increasing eCT and sCT concentrations after incubation in bone slices for 3 days（ P 〈 0.01 ）. There is no significant difference between the Elcatonin group and the Miacalcic group in each concentration（ P 〉 0.05）. Conclusions There is no significant difference between Elcatonin and Miaealcic in inhibiting the osteoclastic proliferation and bone resorption of osteoclasts.

关 键 词：降钙素 益钙宁 密盖息 破骨细胞 

分 类 号：R681[医药卫生—骨科学]