基质细胞衍生因子预处理的内皮祖细胞对大鼠急性心肌梗死的治疗作用  

Endothelial progenitor cells pretreated ex vivo with SDF-1 improve their therapeutic efficiency for acute myocardial infarction

作　　者：王朗[1] 江洪[1] 陈思思[1] 张元元[1] 朱丽华[1] 

机构地区：[1]武汉大学人民医院心血管内科,武汉430060

出　　处：《中华急诊医学杂志》2009年第2期161-164,共4页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金资助项目（30770849）;湖北省自然科学基金资助项目（2005ABA124）

摘　　要：目的探讨基质细胞衍生因子（stromal cell-derived factor-1，SDF-1）预处理的内皮祖细胞（endothelial progenitor cells，EPCs）对大鼠急性心肌梗死的治疗作用。方法培养大鼠骨髓来源的内皮祖细胞，检测SDF-1在体外对EPCs迁移和存活能力的影响。建立大鼠急性心肌梗死模型，随机分为SDF-1＋EPCs组、EPCs组和对照组，每组12只。心梗后24h，将SDF-1预处理或未处理的EPCs经尾静脉注入动物体内，对照组注射不含细胞的培养基。细胞输注后的14d和28d，分别检测血管密度、心功能及心肌梗死面积等指标。结果细胞输注后14d，SDF-1＋EPCs组血管密度高于EPCs组及对照组。细胞输注后28d，SDF-1＋EPCs组大部分心功能指标优于EPCs组和对照组，心梗面积低于EPCs组和对照组。结论SDF-1预处理能促进EPCs的迁移和存活，促进体内EPCs介导的新血管生成，减少心肌梗死面积，促进心功能的恢复。Objective To investigate the therapeutic efficiency of intravenous implanted endothelial progenitor cells （EPCs） pretreated with stromal cell derived factor-1 （SDF-1） in rat model of acute myocardial infarction （AMI）. Method The bone-warrow derived EPCs were cultured. The effects of SDF-1 on the migration and survival of EPCs were evaluated in vitro. The rat models of AMI were produced and randomly divided into SDF-1 ＋ EPCs group （ n = 12）, EPCs group （ n = 12） and control group （ n = 12）. EPCs pretreated with or without SDF- 1 were infused via tail vein 24 hours after AMI modelled. The EBM-2 culture medium without cell was infused in control group. Vessel density, cardiac function and infarct area were measured on 14 days and 28 days after cell implantation. Results Fourteen days after cell implantation, the vessel density of SDF-1 ＋ EPCs group was higher than that of EPCs group and control group. Twenty-eight days after cell implantation, the cardiac function of SDF-1 ＋ EPCs group was better than that of EPCs group and control group. Conclusions EPCs pretreatment with SDF-1 can improve the survival and migratory capacity of EPCs, and increase the therapeutic efficiency for myocardial infarction.

关 键 词：基质细胞衍生因子 内皮祖细胞 急性心肌梗死 预处理 

分 类 号：R542.22[医药卫生—心血管疾病] R457.7[医药卫生—内科学]