不同剂量卡维地洛对大鼠冠状动脉微栓塞后心室重塑的影响  

Effects of different doses of carvedilol on the prevention of left ventricular remodeling following rat coronary microembolization

作　　者：王伟伟[1] 陈良龙[1] 

机构地区：[1]福建医科大学附属协和医院心内科福建省冠心病研究所,福州350001

出　　处：《临床心血管病杂志》2009年第2期145-148,共4页Journal of Clinical Cardiology

基　　金：福建省科技厅科研基金资助项目(No:2005Y031)

摘　　要：目的:比较不同剂量卡维地洛对大鼠冠状动脉微栓塞(CME)后心室重塑的影响。方法:利用大鼠自体的血栓微粒造成心肌内小冠状动脉栓塞,建立CME模型。40只大鼠随机分成4组,即假手术组(SO组)、CME组、小剂量卡维地洛组(LCAR组,1.0 mg·kg-1·d-1)和大剂量卡维地洛组(HCAR组,10.0 mg·kg-1·d-1)。灌胃4周后,行心功能检测、血流动力学测定及心肌病理学分析。结果:与SO组比较,CME组细胞间质胶原容积分数(CVF)与心肌细胞凋亡率(Rapo)明显增加,左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)明显增大,左心室短轴缩短率(LVFS)、左心室射血分数(LVEF)明显降低(均P<0.01);左心室舒张末期压(LVEDP)明显增加,左心室收缩压(LVSP)和左心室腔内压力最大上升速率(+LVdp/dt max)显著下降(均P<0.01)。与CME组比较,LCAR组和HCAR组心肌间质CVF和Rapo显著降低,LVEDD、LVESD明显减小,LVFS明显上升,LVEF明显改善(均P<0.01);LVEDP明显下降,+LVdp/dt max显著上升,心率明显减慢(均P<0.01)。除LVSP外,以上改变HCAR组均较LCAR组明显(P<0.05)。结论:①大鼠CME后心脏发生慢性重塑;②卡维地洛剂量依赖性地改善CME后心室重塑。Objective：To observe the effect of carvedilol at different dose on left ventricular remodeling following rat coronary microembolization. Method：We created a rat model of coronary microembolization （CME） by injecting a suspension of microthrombotic particles generated from the rat clots into left ventricle when clamping the ascending aorta. Forty SD rats were randomly divided into four groups, each consisted of ten rats： sham-operation group （SO）, CME model group （CME）, low dose earvedilol intervention group （LCAR, 1.0 mg· kg^-1· d^-1） and high-dose carvedilol intervention group （HCAR, 10.0 mg · kg ^-1 · d ^-1）. Carvedilol was administered by direct gastric garage for four weeks. Ventricular function and hemodynamic studies were performed, and then pathologic analysis was performed. Result： Compared with group of SO, both collagen volume fraction （CVF） and Rapo （apoptosis rate） were increased, and both left ventricular end-diastolic diameter （LVEDD） and left ventrieular end-systolic diameter （LVESD） were increased in group of CME, but left ventricular fraction shortering （LVFS） and left ventricular ejection fraction （LVEF） were reduced （P〈0.01, each）. Compared with group of SO, both left ventricular systolic pressure （LVSP） and left ventricular maximum positive dp/dt （＋LVdp/dtmax） were decreased in group of CME （P〈0.01 ,each）, but LVEDP was increased （P〈0.01）. Compared with group of CME, both CVF and Rapo were decreased, LVEDD and LVESD were smaller （P〈0.01, each）, LVEF and LVFS was higher （P〈0.01 ,each）, ＋LVdp/dtmax were increased and LVEDP was decreased （P〈0.01, each）, heart rate were slowed down （P〈0.01 ,each） in the two groups of different doses 28 days after operation. The above-mentioned changes were more remarkable in group of HCAR compared to group of LCAR （P〈0.05）. Conclusion： ①Left ventricle is undergoing progressive remodeling after CME. ②Carvedilol dose-dependently prevent ventricul

关 键 词：冠状动脉微栓塞 心室重塑 卡维地洛 

分 类 号：R331.3[医药卫生—人体生理学]