新型壳聚糖微球／磷酸钙骨水泥的动物体内植入研究  

Animal implantation with a new type of chitosan microspheres/calcium phosphate cement

作　　者：孟丹[1,2] 谢秋菲[1] 

机构地区：[1]北京大学口腔医学院.口腔医院修复科,北京100081 [2]首都医科大学附属北京口腔医院修复科

出　　处：《北京大学学报（医学版）》2009年第1期80-85,共6页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金项目(30772447)资助~~

摘　　要：目的:通过动物实验观察分析新型的、可注射壳聚糖微球/磷酸钙骨水泥的生物相容性、生物降解性、成骨特性及其临床可操作性。方法:12只新西兰大白兔的股骨髁部钻取直径4 mm、深6 mm的洞造成骨缺损,每只大白兔左右两侧股骨分别注入实验材料壳聚糖微球/磷酸三钙骨水泥和对照材料——α-磷酸三钙骨水泥(α-trical-cium phosphate cement,α-TCP),术后第3天摄X线片观察骨水泥充填情况。实验动物分成3个时点组,每组4只,分别在术后第8、16、24周取实验侧壳聚糖微球/磷酸三钙骨水泥和对照侧α-磷酸三钙骨水泥的植入处标本。HE染色后光镜下观察骨水泥在骨内的降解及新骨再生情况。扫描电镜观察骨-骨水泥界面的超微结构。结果:壳聚糖微球/磷酸三钙骨水泥第8周时有少量降解,第16周时降解吸收较多,壳聚糖微球降解后在骨水泥内部原位形成了大小不等的孔隙。24周时材料大量降解,仅存少量大粒径壳聚糖微球,降解同时有大量新骨生成。α-TCP骨水泥较壳聚糖微球/磷酸三钙骨水泥降解较少,骨水泥内部致密少孔。结论:壳聚糖微球/磷酸三钙骨水泥在骨组织内具有良好的生物相容性和骨结合能力。在骨修复过程中,较对照材料α-TCP骨水泥降解速度快,并可引导新骨生成。Objective： To investigate bioactivity, biodegradation, bone conductive properties, and clinical maneuverability of a novel injectable chitosan microspheres/calcium phosphate cement （CPC）. Methods： The bone defect of π × 4 mm × 4 mm × 6 mm was made at both thighbones of 12 rabbits, and experimental material （chitosan microspheres/CPC ） or control material （α-tricalcium phosphate cement, α-TCP） was injected into the defect respectively. The filling situation was evaluated by X-ray 3 days after operation. The rabbits were divided into three groups, four for each group. The samples with chitosan microspheres and the control with α-TCP were collected at 8, 16, 24 weeks after operation. Histological examination and scanning electron microscope （SEM） evaluation were performed. Results： The absorption of implants with chitosan microspheres was observed at 8 weeks, and became more apparent at 16 weeks. Different size of cavities were observed in CPC after the degradation of chitosan microspheres. The chitosan mierospheres/CPC was dramatically degradated after 24 weeks with a few chitosan mierospheres, and new bone replaced the degradated materials. The implants with α-TCP were absorbed slowly compared with the chitosan mierospheres/CPC. The cavities in α-TCP were small. Conclusion： The ehitosan microspheres/calcium phosphate cement has the characters of good biocomptable and osteocombinative ability. Compared with the control material, adding chitosan microspheres into CPC could enhance its degradability and facilitate the new bone formation.

关 键 词：磷酸钙类 骨水泥 壳聚糖 动物实验 

分 类 号：R68[医药卫生—骨科学]