吸毒者尿液中吗啡的现场薄层检测法(第二报)  

The Present TLC method for Determination Morphine in the Urine of Drug Addicts

作　　者：张薇薇[1] 林明美[1] 王瑛[1] 董皓 郝仙娣 

机构地区：[1]北京市临床药学研究所

出　　处：《中国药物滥用防治杂志》1998年第2期26-37,共12页Chinese Journal of Drug Abuse Prevention and Treatment

摘　　要：本文在第一报〔１〕的基础上，对实验条件，方法的灵敏度和２９种有关药物及空白尿对方法的干扰情况等进行了系统考察；同时与ＧＣ－ＭＳ和试剂盒进行方法比较；对吗啡衍生物（ＲＳＯ２－Ｍ）进行结构分析；临床验证２４７例。方法灵敏度为１０ｎｇ（板上）；ＳＩ展开剂的平均Ｒｆ值为０．２９，ＳＤ为０．０２４；ＳⅡ展开剂的平均Ｒｆ值为０．３８，ＳＤ为０．０７２；酸水解的最佳条件为１００℃加热３０分钟，衍生化的最佳条件为ｐＨ８－９，７０℃水浴１０分钟；除可待因，蒂巴因外其余２６种相关药物和空白尿对方法均无干扰；定性结果与ＧＣ－ＭＳ完全一致，半定量结果与ＧＣ－ＭＳ具有一定可比性；临床验证结果满意。On the basis of the first report,the conditions of the experiment,the sensitivity of the method,the interference of the 29 kinds of concerned drugs and the normal urine were described in this report.Our method compared with GC-MS and Surestep TM Drug Screen (morphine).The clinical test were 247 instances.The results showed that the limit of detection is 10ng(on the silica gel plate),the R f of the first solvent is 0.29 and the SD is 0.024,the R f of the second solvent is 0.38 and the SD is 0.072.It is the best conditions of the urine acid hydrolysis that the urine are heated at 100℃ for 30 minutes;It is the best conditions of the derivation that the urine are heated at pH8-9 and 70℃ for 10 minutes in waterbath.All 26 kinds of the drugs and the normal urine did not interfer with the method,except codeine and thebaine.The results of qualitative were same as GC-MS and the results of semiquantitative were proportionate with GC-MS.The results of clinical test were most satisfactory.

关 键 词：吗啡 薄层检测法 吸毒者 尿液 海洛因依赖者 

分 类 号：R595.504[医药卫生—内科学]