转Na^＋／H^＋逆向转运蛋白(PeNhaD1)基因派间杂种110杨的获得  

Genetic Transformation of PeNhaD1 Gene to the Hybrid Poplar 110

作　　者：陈彩霞[1,2] 司瑞新 沈昕[1] 陈少良[1] 张华[1] 李静[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京市大东流苗圃,北京102211

出　　处：《西北林学院学报》2009年第1期61-65,共5页Journal of Northwest Forestry University

基　　金：国家自然科学基金重点项目(30430430);863计划项目(2006AA10Z131);全国高校优秀博士学位论文作者专项基金(200152);教育部高等学校优秀青年教师教学科研奖励计划(2002-323)

摘　　要：胡杨Na+/H+逆向转运蛋白基因(PeNhaD1)编码一种质膜Na+/H+逆向转运蛋白,在胡杨细胞拒盐机制中具有重要作用。本研究利用根癌农杆菌(Agrobacterium tumefacience)介导的叶盘法将PeNhaD1基因转入盐敏感且速生的派间杂种110杨(P.deltoides×P.maximowiczi‘Eridano’)中。经PCR和PCR-Southern检测已获得转PeNhaD1基因的110杨再生植株。The PeNhaD1 gene isolated from Populus euphratica, encoded a plasma Na^＋/H^＋ antiporter protein has an important value in the salt-tolerant mechanism of P. euphratica. In this research, PeNhaD1 gene was transformed to the salt-sensitive and fast-growing hybrid poplar 110 （P. deltoides× P. maximowiczii cv. ‘Eridano＇） via Agrobacterium turnefacience mediated transformation system. The result of PCR amplification and PCR-Southern hybridization showed that PeNhaD1 gene had been integrated into the genome of the transgenic hybrid poplar 110 plants.

关 键 词：PeNhaD1基因 基因转化 派间杂种110杨 

分 类 号：S722.36[农业科学—林木遗传育种]