饱和白光引起的光系统Ⅱ捕光复合体(LHCⅡ)从反应中心复合体脱离不同于弱红光引起的状态1向状态2的转换(英文)  

Dissociation of Photosystem Ⅱ Light-harvesting Complex(LHCⅡ) from the Reaction Center Complex Induced by Saturating White Irradiation Differs from the Transition from State 1 to State 2 Induced by Weak Red Irradiation

作　　者：陈悦[1,2] 许大全[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032 [2]中国科学院研究生院,北京100049

出　　处：《云南植物研究》2009年第1期67-74,共8页Acta Botanica Yunnanica

基　　金：The National Basic Research Program of China (Project No.2005CB121106)

摘　　要：我们观测了不同光照预处理对拟南芥、小麦和大豆叶片光合作用和低温(77K)叶绿素荧光参数F685、F735和F685/F735的影响。野生型拟南芥叶片光合作用对饱和光到有限光转变的响应曲线是V型,而缺乏叶绿体蛋白激酶的突变体STN7的这一曲线为L型。饱和白光可以引起拟南芥叶片F685/F735的明显降低,但是F735没有明显增高,而弱红光可以导致拟南芥叶片F685/F735的明显降低和F735的明显增高,表明弱红光可以引起状态1向状态2的转变,同时伴随从光系统Ⅱ脱离的LHCⅡ与光系统I的结合,而饱和白光只能引起LHCⅡ从光系统Ⅱ反应中心复合体脱离。并且,低温叶绿素荧光分析结果证明,饱和白光可以引起大豆叶片LHCⅡ脱离,但是不能引起小麦叶片LHCⅡ脱离,而弱红光可以引起小麦叶片的这种状态转换,却不能引起大豆叶片的这种状态转换。因此,饱和白光引起的野生型拟南芥和大豆叶片的LHCⅡ脱离不是一个典型的状态转换现象。The effects of different irradiance pre-treatments on leaf photosynthesis and low temperature （77K） chlorophyll fluorescence （LTCF） parameters F685, F735, and F685/F735 were observed in Arabidopsis, wheat, and soybean leaves. The curve of photosynthetic responsing to irradiance transition froro saturating to limiting one in Arabidopsis wild-type leaves was the V pattern, while the curve in Arabidopsis mutant lacking chloroplast protein kinase STN7 was the L pattern. Saturating white irradiation （SWI） could induce the significantly decreased F685/F735 without a significantly increased F735, while weak red irradiation （WRI） could lead to the significantly declined F685/F735 with a significantly increased F735. The results showed that the attachment of dissociated LHC Ⅱ to PS Ⅰ in Arabidopsis wild-type leaves, indicating that WRI can cause the transition from state 1 to sate 2, while SWI can only induce LHC Ⅱ dissociation from PSⅡ reaction center complex. Moreover the results of LTCF analysis demonstrated that SWI could induce the LHC Ⅱ dissociation in soybean but not wheat leaves, while WRI could cause the state transition in wheat but not soybean leaves. Hence the LHCⅡ dissociation caused by SWI in Arabidopsis wild-type and soybean leaves was not a typical phenomenon of the state transition.

关 键 词：拟南芥 光系统Ⅱ捕光复合体(LHC Ⅱ) 低温(77K)叶绿素荧光 净光合速率 可逆脱离 状态转换 

分 类 号：Q945.11[生物学—植物学]