参附对单侧尿路梗阻大鼠肾脏白介素18表达的影响  

Effect of Shenfu injection on the expression of Interleukin-18 in rat kidney after unilateral ureteral obstruction

作　　者：陈文莉[1] 黄艳霞 郑宇明[1] 邵菊芳[3] 

机构地区：[1]湖北省武汉市中心医院肾内科,湖北武汉430014 [2]广州经济技术开发区医院 [3]华中科技大学同济医学院附属同济医院肾内科

出　　处：《中国医师杂志》2009年第1期24-27,共4页Journal of Chinese Physician

基　　金：湖北省武汉市卫生局2006年度临床重点学科研究资助项目（2006-11）

摘　　要：目的研究单侧尿路梗阻后大鼠肾脏白介素18（IL-18）的表达及中药参附对其的影响。方法采用单侧输尿管结扎（UUO）制造梗阻性肾病模型，将56只大鼠随机分为对照组（假手术组）、手术组（UUO组）和治疗组（UUO＋参附），术后7、14d观察肾组织病理改变，检测肾组织中IL—18的表达。结果与对照组相比，手术组肾间质出现了明显的纤维化，IL-18的表达在术后第7天明显增加，第14天表达更强，与手术组相比，治疗组肾间质纤维化明显减轻，而治疗组与手术组同一时闽点相比较，IL-18的表达均有所降低，差异有统计学意义（P〈0．05）。结论参附可以下调肾组织IL-18的表达，减轻肾小管-间质纤维化，发挥肾保护作用。Objective To study the expression of interleukin-18 （IL-18） during the progression of renal interstitial fibrosis in rat kidney after unilateral ureteral obstruction（UUO） and the effect of Shenfu injection. Methods The obstructive nephropathy model was established by unilateral ureteral obstruction（UUO）. Fifty-six rats were randomly assigned into shame operation group, operation group（UUO group） and treatment group （ UUO ＋ Shenfu）. After 7 and 14 days, the renal function and histopathological changes were evaluated. Immunohistochemistry was used to examine the expression of IL - 18 in renal tissue. Results In comparison with the shame opeartion group, the operation group showed obvious renal interstitial fibrosis, and the expression of IL-18 increased significantly（ P 〈 0. 05 ）. Compared with the operation group, the degree of interstitial fibrosis was obviously ameliorated in the treatment group, and the expression of IL-18 decreased significantly after treatment for 7 days （ P 〈 0. 05 ）, and decreased more after treatment for 14 days （ P 〈 0.05 ）. Conclusions Shenfu injection may protect renal function by decreasing the expression of IL-18 in the progression of renal interstitial fibrosis.

关 键 词：参附汤/药理学 尿道梗阻/中药疗法/病理学 白细胞介素18 

分 类 号：R285.5[医药卫生—中药学]