利心Ⅰ号对实验性心力衰竭大鼠心肌组织MMP-3 TIMP-1表达的影响  

Effect of Lixin Ⅰ on Rats Model with Adriamycin:Expression of MMP-3 TIMP-1 in rats' myocardia

作　　者：刘莉[1] 金娟[1] 窦金金[1] 

机构地区：[1]黑龙江中医药大学第一附属医院,黑龙江哈尔滨150040

出　　处：《辽宁中医杂志》2009年第2期187-188,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:基于对CHF基本病机的认识和大量的临床观察,采用先进的实验手段和方法,研究利心I号对大鼠心肌组织MMP-3、TIMP-1表达的影响。方法:利用阿霉素的心肌毒性复制CHF的动物模型。将84只Wistar雄性大鼠随机平均分为6组:正常对照组、模型组、依那普利对照组、心宝对照组、利心I号低剂量组、利心I号高剂量组。采用半定量逆转录聚合酶链反应(RT-PCR)的方法测定大鼠心肌组织MMP-3、TIMP-1的表达。结果:利用阿霉素的心肌毒性可以成功复制CHF的动物模型;利心I号能够显著降低CHF大鼠心肌组织MMP-3表达而升高TIMP-1的表达。结论:利心I号能够抑制或延缓心室重塑、改善心功能,是治疗CHF的有效方剂。Objective：To observe the influence of LiXin Ⅰ on MMP-3,TIMP- 1 of the CHF rats model with Adriamycin. Methods：Adriamycin is applied to build CHF model. Eighty-four male wistar rats were randomly divided into six groups：normal control group, model group, enalapril control group, XinBao control group, high-dose LiXin Ⅰ group, low-dose LiXin Ⅰ group. MMP3, TIMP - 1 expression of the rats＇ myocardia are measured with RT - PCR. Results ： To build CHF model with injecting Adriamyein into the rats＇ abdomen can injure their myocardia. It can decrease MMP - 3 expression and increase TIMP - 1 expression obviously. Conclusion：LinXin Ⅰ is effective in the treatment of CHF and can restrain or delay ventricular remodeling and improving heart function.

关 键 词：利心Ⅰ号 心力衰竭 心室重塑 

分 类 号：R541.6[医药卫生—心血管疾病]