从塔里木兔体内首次分离出婴儿利什曼原虫  

Leishmania infantum firstly isolated from Yarkend hare(Lepus yarkandensis)

作　　者：廖力夫[1] 燕顺生[1] 乌守巴特[1] 吴敏[2] 徐兵[1] 张勇[2] 侯岩岩[1] 雷刚[1] 

机构地区：[1]新疆维吾尔自治区疾病预防控制中心新疆实验动物研究中心,乌鲁木齐830002 [2]浙江大学生命科学学院

出　　处：《中国媒介生物学及控制杂志》2009年第1期45-47,共3页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(30460120)

摘　　要：目的调查塔里木盆地荒漠型黑热病的保存宿主。方法开展动物与传播媒介及其环境的调查。冬季采集动物样品,用ELISA筛选抗体阳性动物;接种草原兔尾鼠和液体培养基培养分离利什曼原虫;分子生物学方法测定从当地黑热病患者、动物和传播媒介分离出的利什曼原虫特定基因序列。结果塔里木兔和家犬有抗利什曼原虫抗体;从44只抗体阳性塔里木兔中分离出3株利什曼原虫;塔里木兔、黑热病患者和吴氏白蛉分离出的利什曼原虫测定的NAGT核基因序列相同,与GenBank注册的婴儿利什曼原虫AF205934一致。结论塔里木兔是塔里木盆地荒漠型黑热病的野生宿主之一。Objective To survey reservoir hosts of desert-type kala-azar in Tarim Basin. Methods Collect animal samples in winter, and screen the samples which antibody were positive by ELISA. Leishmania was isolated by Lagurus lagurus inoculation and tissue culture. The specific gene sequences of Leishmania infantum isolated from kala-azar patients, Yarkend hare and Phlebotomus wui were amplified by molecular biological technology. Results There was Leishmania antibodies in Yarkend hare and Canis familiaris, and 3 strains of Leishmania were isolated from 44 of Yarkend hare which antibodies were positive. The NAGT gene sequences amplified from hares （3 strains）, patients （1 strain） and vectors （6 strains） were same, according with the sequence of L.infantum （AF205934） from GenBank. Conclusion It suggests that yarkend hare is one of the primal host of desert-type kala-azar in Tarim Basin.

关 键 词：婴儿利什曼原虫 荒漠型黑热病 塔里木兔 吴氏白蛉 宿主 

分 类 号：R382.22[医药卫生—医学寄生虫学]