二硫化碳对神经细胞混悬液及突触膜ATP酶和胆碱酯酶活性影响  

Effects of Carbon Disulfide on Membrane-Bound Integral Enzymes with Neuron and Glia Cell Mixture and Synaptosome

作　　者：高艳华[1] 梁友信[1] 

机构地区：[1]上海医科大学劳动卫生教研室

出　　处：《劳动医学》1998年第1期1-3,共3页

基　　金：上海市科研基金

摘　　要：本研究目的在于寻求神经性毒物体外的测试方法，并对ＣＳ２的毒作用机制进行探讨。选用神经细胞混悬液及超速密度梯度离心分离的突触膜为模型，观察ＣＳ２不同染毒浓度对Ｎａ＋－Ｋ＋－ＡＴＰ酶及胆碱酯酶活性的影响。结果表明，ＣＳ２对Ｎａ＋－Ｋ＋－ＡＴＰ酶有明显的抑制作用，而对胆碱酯酶活性无影响。推测Ｎａ＋－Ｋ＋－ＡＴＰ酶在ＣＳ２的毒作用机制中起一定作用，突触膜可作为研究预测神经性毒物的毒性潜力。To observe the influence of CS2 on activity of Na+K+ATPase and AchE,the neurons and glia cell mixture obtained from newborn rats and the synaptosome prepared by density gradient centrifugation were employed models.The significant inhibition of Na+K+ATPase activity was observed (p<0.05). The Na+K+ATPase activity was lowered down to 60% of the normal at a 40μM level of CS2.No significant effect was seen for AchE.

关 键 词：二硫化碳 神经细胞混悬液 ATP酶 胆碱酯酶 毒理 

分 类 号：R995[医药卫生—毒理学] R136.31[医药卫生—药学]