重组人白细胞介素-6包涵体精制工艺研究  

Study on the purification process of rhIL-6 inclusion body

作　　者：饶海林 曾海鹏 张珂 袁涛 张雪梅 

机构地区：[1]成都生物制品研究所生物技术研究室,610023

出　　处：《国际生物制品学杂志》2009年第1期1-3,共3页International Journal of Biologicals

基　　金：四川省科技厅科技项目（05SG022-019）

摘　　要：目的建立重组人白细胞介素-6（recombinant human interleukin-6，rhIL-6）包涵体精制工艺，获取高纯度的包涵体蛋白。方法采用超声破碎法和生物酶解法破碎细胞；摸索洗涤缓冲液中尿素的适当浓度，经多次洗涤获取高纯度的包涵体蛋白。结果成功建立了rhIL-6包涵体蛋白的精制工艺，其中尿素浓度为2．0mol／L，溶菌酶浓度达到0．8mg／g菌体。包涵体蛋白洗涤后纯度达到90％以上。结论建立合理的精制工艺，包涵体蛋白纯度达到90％以上。Objective To establish the purification process of recombinant human interleukin-6 （ rhIL- 6） inclusion body and obtain more purified inclusion body. Methods The bacterial cells were broken by the methods of uhrasonication and enzyme digestion, the appropriate concentration of the urea was found out, and more purified inclusion bodies were obtained by washing inclusion bodies more than once. Results The purification process of rhIL- 6 inclusion body was established. The concentration of the urea was 2.0 mol/L and the concentration of lysozyme was 0.8 mg/g bacteria. The purity of inclusion body obtained could come up to more than 90%. Conclusions The reasonable purification process is established, and the purity of rhIL-6 inclusion body can reach more than 90%.

关 键 词：包涵体 RHIL-6 生物酶解法 超声破碎法 

分 类 号：TQ[化学工程]