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作 者:袁小青[1,2] 马向华[1] 丁亚琴[1] 王芳[1] 沈捷[3]
机构地区:[1]南京医科大学第一附属医院内分泌科,江苏南京210029 [2]常州市中医院,江苏常州213003 [3]南京医科大学第一附属医院中心实验室,江苏南京210029
出 处:《南京医科大学学报(自然科学版)》2009年第2期161-165,共5页Journal of Nanjing Medical University(Natural Sciences)
摘 要:目的:研究大黄酸(rhein,Rh)对人前体脂肪细胞增殖与分化的影响,并进一步探讨其作用机制。方法:采用噻唑蓝(MTT)比色法测定RH对人前体脂肪细胞增殖的影响;通过形态学观察脂肪细胞的分化程度及形态学变化;并用油红O染色法,检测脂肪细胞内甘油三酯积聚量的变化;应用RT-PCR检测RH干预后分化抑制基因CHOP mRNA的表达。结果:各浓度组RH(0.1、1、2.5、5、10ug/ml)抑制人前体脂肪细胞增殖,作用呈剂量依赖性;RH对脂肪细胞分化的抑制作用亦呈剂量依赖性;1ug/ml的RH使CHOP mRNA表达增加。结论:RH可抑制人前体脂肪细胞增殖与分化,该作用可能与CHOP表达上调有关。Objective:To explore the effects of Rhein(RH) on proliferation and differentiation of human preadipocytes. Methods: Omental adipose tissue biopsies were obtained from 15 patients who were undergoing elective open-abdominal surgery. The primary culture and differentiation induction of human preadipocytes were performed, and the human preadipocytes were treated with rhein at different concentrations at proper moments. Dynamic morphological changes of the human preadipocytes were observed, and their proliferation and differentiation were assessed with Colorimetric MTY Assay and Oil Red O Staining. The expression of CHOP(a gene regulating the differentiation of preadipocytes)was.detected by RT-PCR. Results:After 24 hours with RH, 2.5 ug/ml RH decreased the MTY values of the human preadipocytes by 35.5% (P 〈 0.05), compared with the control group without RH. The human preadipocytes with RH cumulated less adipose in the endochylema than those without RH obviously. 2.5 ug/ml RH decreased the differentiation degree of the human preadipocytes differentiated 8-10 days by 66.2%(P 〈 0.05). 1 ug/ml RH increased the mRNA level of CHOP obviously compared with the control group. Conclusion:Rhein may Significantly inhibit the proliferation and differentiation of the human omental preadipocytes, which would contribute to the increased expression of CHOP gene.
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