橡胶树高效农杆菌转化体系的建立  

Establishment of a Highly Efficient Agrobacterium-Mediated Transformation System for Hevea brasiliensi

作　　者：赵辉[1] 赵润江[2] 陈雄庭[1] 彭明[1] 

机构地区：[1]中国热带农业科学院,海南儋州571737 [2]北京林业大学园林学院,北京100083

出　　处：《热带作物学报》2008年第6期678-683,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目《橡胶抗寒转基因种质研究》(批准号:30460114)资助。

摘　　要：在橡胶(Hevea brasiliensis)花药组织培养和胚性愈伤长期继代增殖技术的基础上,分别以巴西橡胶热研7-33-97品种的花药脱分化愈伤组织和经继代增殖的花药胚性愈伤组织为转化受体,进行橡胶农杆菌转化体系比较研究。结果表明:胚性愈伤组织是更适宜的转化受体。On the basis of Hevea brasiliensis anth er culture and embryonic calli long-term subculture, the anther calli and long-term subculture embryonic calli of Reyan 7-33-97 were selected as transformation materials. The growth of the different transformation materials was compared to establish a new way for transformation of Hevea brasiliensis mediated with Agrobacterium tumefacian. The results show that the embryonic calli have a higher transformation rate than the anther calli.

关 键 词：巴西橡胶树 遗传转化 转化受体 农杆菌介导法 

分 类 号：S794.101[农业科学—林木遗传育种]