苦楝丛枝病植原体海南株系SecY基因序列分析及亚组分类  

Sequence Analysis of SecY of Phytoplasma from Chinaberrywitches'-broom phytoplasma in Hainan and Is Sub-section Identification

作　　者：罗大全[1,2] 车海彦[1,2] 刘先宝[1,2] 符瑞益[1,2] 叶莎冰[1,2] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737 [2]海南省热带农业有害生物检测监控重点实验室,海南儋州571737

出　　处：《热带作物学报》2008年第6期799-802,共4页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(项目批准号:30660103)资助。

摘　　要：利用植原体16S rⅠ组通用引物对secYF1/secYR1,应用PCR技术从采自海南省儋州地区的表现典型丛枝症状的苦楝植株总DNA中扩增到约1.4 kb的特异片段,将此片段克隆后进行序列测定,序列分析及系统关系树构建的结果表明,该片段长1 358 bp,苦楝丛枝病海南株系(Chinaberry witches'-broom phytoplasma strain Hainan,CWB-Hn)与玉米丛矮(Maize bushy stunt,MBS)植原体聚类在同一条进化枝上;AluⅠ,MseⅠ和Tsp509Ⅰ这3种酶的电子酶切图谱表明,CWB-Hn与MBS的酶切图谱一致,故初步判定苦楝丛枝病植原体海南株系属于secY-L亚组,在亚组水平上进一步明确了苦楝丛枝病植原体海南株系的分类地位。The samples of witches＇-broom from Chinaberry tree were collected in Danzhou, Hainan Province. By using universal primers （secYF1/secYR1） for phytoplasma secY, a fragment of about 1.4 kb was amplified in DNA samples extracted from diseased Chinaberry tree. Phylogenetic analysis based on amino acid sequences of the secY gene from the Chinaberry witches＇-broom strain Hainan and representative phytoplasma strains in the 16SrⅠ group showed that Chinaberry witches＇-broom phytoplasma strain Hainan （CWB-Hn） and maize bushy stunt （MBS） was in the same clade. The PCR-amplified secY fragments were digested in silico with 3 distinct restriction enzymes （AluⅠ, MseⅠ and Tsp509Ⅰ ） for RFLP analysis. The resultant deduced restriction map suggested that restriction enzyme mapping of CWB-Hn was consistent with that of MBS. The results indicated that CWB-Hn belongs to secY- L subgroup.

关 键 词：苦楝丛枝病 植原体 海南株系 SECY 亚组分类 

分 类 号：S718.87[农业科学—林学]