白术茯苓汤调控脾虚大鼠血管活性肠肽总多糖的提取工艺研究  

Study on Extraction of the Polysaccharide in Controlling Rat Spleen Deficiency Vasoactive Intestinal Peptide from Atractylodes Poria Soup

作　　者：彭腾[1] 贾波[1] 彭成[1] 文跃强[1] 宴永明[1] 张楠[1] 贺小芳[1] 

机构地区：[1]成都中医药大学,成都610075

出　　处：《成都医学院学报》2008年第4期253-255,268,共4页Journal of Chengdu Medical College

基　　金：国家自然科学基金资助项目(编号30572296)

摘　　要：目的研究白术茯苓汤总多糖的提取工艺。方法通过单因素实验及正交实验确定提取总多糖的优化条件,用分光光度法测定总多糖的含量。结果白术茯苓汤总多糖提取的最佳条件是:料液比为1∶30、提取时间为2.0h、提取次数为3次、温度90℃.在此条件下测得白术茯苓汤中总多糖含量为48.25mg/g.结论该方法简便、快速、准确,适用于该复方中总多糖的含量测定。Objective To investigate the extraction process of polysaecharide from Atractylodes Poria soup. Methods The optimal condition was determined by the orthagonal experiment and single-factor test. Polysaecharides were measured by spectrophotometry. Results Experiment reveals that optimal condition for extraction was as fol lows： temperature： 90℃; duration： 2 hours; times： 3 times; ratio： 1：30（w/v）. The volume of polysaccharides in Atractylodes Poria soup is 48.25 mg/g. Conclusion The method is simple, rapid and accurate for determination of polysaceharide from the compound.

关 键 词：白术茯苓汤 总多糖 正交实验 含量测定 

分 类 号：R284.2[医药卫生—中药学]