SDS—聚丙烯酰胺凝胶电泳分析壳寡糖  

Electrophoretic Analysis of Chitooligosaccharide by SDS-PAGE

作　　者：王亮[1] 吴昌英[1] 刘桦[2] 张涛 

机构地区：[1]成都医学院基础医学院,生物技术专业成都610083 [2]成都医学院基础医学院生物技术实验室,成都610083

出　　处：《成都医学院学报》2008年第4期263-265,共3页Journal of Chengdu Medical College

基　　金：四川省教育厅青年基金资助项目(项目编号2006B112)

摘　　要：目的研究一种快速准确的分析壳寡糖组成的定性测定方法。方法通过连续电泳和不连续电泳对比实验确定壳寡糖的最佳分析条件。结果浓缩胶浓度为3%,分离胶浓度为17.5%,上样量为10μl,恒定电流为40mA,电泳时间50min条件下,获得最佳壳寡糖分析图谱。结论SDS-聚丙烯酰胺凝胶电泳分析壳寡糖组成的方法的确是一种简单快速有效地定性检测方法。Objective To explore a qualitative, fast and accurate way for analyzing composition of chitooligosaccharide. Methods By consecutive electrophoresis and non-consecutive electrophoresis experiments, the best analysis conditions of chitooligosaccharide were determined. Results The concentration of plastic was 3 percent, the concentration of separation of plastic was 17.5 percent, the volume of samples was 10ul, constant current was 40 mA , electrophoresis time was 50 min. Best analysis photos of chitooligosaccharide Can be obtained under those conditions. Conclusion The method of analyzing composition of chitooligosaccharide is truly a simple, effective, accurate and qualitative way.

关 键 词：壳寡糖 电泳分析 聚丙烯酰胺凝胶电泳 

分 类 号：Q532.5[生物学—生物化学]