凝固酶阴性葡萄球菌及其ica操纵子检测的临床研究  

Coagulase-negative Staphylococci and ica Operon Examination:A Clinical Study

作　　者：梁冰[1] 叶嗣颖[2] 于红[3] 顾大勇[1] 姜美娟[1] 王华强[1] 刘妮妮[1] 

机构地区：[1]解放军第401医院检验科,山东青岛266071 [2]华中科技大同济医学院微生物教研室,湖北武汉430030 [3]青岛大学医学院微生物教研室,山东青岛266071

出　　处：《中华医院感染学杂志》2009年第3期241-243,共3页Chinese Journal of Nosocomiology

基　　金：青岛市科技发展计划项目(05-1-NS-76)

摘　　要：目的评价凝固酶阴性葡萄球菌(CNS)及致病物质在临床感染中的意义。方法对医院的114株CNS标本进行菌种鉴定,同时PCR扩增icaD基因。结果表皮葡萄球菌分离率最高(41.2%);分离的CNS,其ica操纵子携带率以深静脉置管(44.4%)、伤口分泌物(42.1%)和血液(36.8%)较高,而呼吸道标本占24.0%,尿液占14.1%;ica操纵子阳性菌株的比率以表皮葡萄球菌最高(42.6%),溶血葡萄球菌为19.0%。结论ica操纵子可出现在多种CNS中,以表皮葡萄球菌为主;CNS存在于不同部位具不同意义,呼吸道和尿标本中检出的CNS应慎重评价,血液中分离的CNS污染的可能性大;以PCR技术扩增ica操纵子,方法简单易行,能够满足临床要求。OBJECTIVE To investigate the significance of CNS in clinical infections. METHODS A total of 114 CNS strains isolated from our hospital were identified by conventional procedures and the icaD gene was amplified by PCR. RESULTS Of all CNS strains, the highest isolated rate was S. epidermidis （41.2%）. CNS isolated from deep venous catheters, wound secretions and blood had a higher rate of carrying ica operon, accounted for 44.4%, 42. 1% and 36.8%, respectively, whereas 24.0% in respiratory secretions and 14.1% in urine. Among the ica operon positive CNS strains, the percentages of S. epidermidis and S. haemolyticus were 42. 6% and 19.0%. CONCLUSIONS There is a wide range of CNS species carrying ica operon, especially in S. epiderrnidis. CNS isolated from different specimens might have different significances. It should be cautious to assess the results of isolated CNS from respiratory and urine specimens. The CNS isolates from blood specimens might be contaminated. The PCR method for the ica operon is simple and easy.

关 键 词：凝固酶阴性葡萄球菌 icaD基因 黏附 感染 

分 类 号：R378[医药卫生—病原生物学]