重症监护病房分离铜绿假单胞菌的耐药性及分子流行病学检测  

Resistance and Molecular Epidemiology of Pseudomonas aeruginosa Isolated from ICU

作　　者：王中新[1] 魏艳艳[2] 熊自忠[2] 

机构地区：[1]安徽医科大学第一附属医院检验科,安徽合肥230022 [2]安徽医科大学第一附属医院感染病科,安徽合肥230022

出　　处：《中华医院感染学杂志》2009年第4期466-467,共2页Chinese Journal of Nosocomiology

基　　金：安徽省自然科学基金(050430804);安徽省优秀青年科技基金(08040106815)

摘　　要：目的了解重症监护病房(ICU)分离铜绿假单胞菌对常用抗菌药物的耐药性及分子流行病学特征。方法采用MicroScan WalkAway-40全自动微生物分析仪对铜绿假单胞菌进行药物敏感性测定,采用随机引物聚合酶链反应(ERIC-PCR)方法进行分子流行病学检测。结果铜绿假单胞菌对头孢他啶的耐药率最低(27.3%)、其次为头孢吡肟(45.5%);对头孢噻肟的耐药率最高达100.0%、其余抗菌药物耐药率均>60.0%;ERIC-PCR结果显示,有5株铜绿假单胞菌属于同一型别。结论ICU分离铜绿假单胞菌多药耐药显著,仅对头孢他啶较为敏感,菌株间存在克隆传播、临床应加强检测和监测。OBJECTIVE To investigate the resistance and molecular epidemiology of Pseudomonas aeruginosa isolated from ICU. METHODS Susceptibility of P. aeruginosa isolates to 14 antimicrobial agents were tested by microScan WalkAway-40 system, and Molecular typing was analyzed by enterobaeterial repetitive intergenic consensus（ERIC-PCR）. RESULTS The lowest resistance rate of P. aeruginosa to the antimicrobial agents was to eeftazidime （27.3%）,then to cefepime （45.5%）. The highest resistance rate was to cefotaxime （100.0%） and the resistance rate to other antimicrobial agents was more than 60.0%. Patterns of ERIC-PCR were same in 5 strains of P. aeruginosa. CONCLUSIONS P. aeruginosa isolated from ICU is resistant to most of antimicrobial agents and clone spread is found in them. We should enhance detection and surveying of these isolates.

关 键 词：铜绿假单胞菌 耐药性 抗菌药物 肠杆菌基因间重复序列聚合酶链反应 

分 类 号：R378.991[医药卫生—病原生物学]