人参皂甙Rg1、肉桂酸与丹参酮ⅡA组合对体外人成骨肉瘤MG-63细胞形态结构和终末分化指标的影响  

Effects of the combination of ginsenoside Rg1,cinnamic acid and tanshinone ⅡA on the morphology and terminal differentiation of MG-63 cells in vitro

作　　者：李祺福[1] 王国红[1] 石松林[1,2] 马宗源[1] 林昌健[2] 荆光军[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学和肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]厦门大学化学化工学院固体表面物理化学国家重点实验室,福建厦门361005

出　　处：《解剖学报》2009年第1期13-19,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30470877,20773100);国家基础研究(973计划)资助项目(2007CB935603);福建省自然科学基金资助项目(2008J0302);中国博士后科学基金资助项目(20070420754)

摘　　要：目的研究中药有效成分人参皂甙Rg1、肉桂酸和丹参酮ⅡA组合（RCT）对人成骨肉瘤MG-63细胞的形态与超微结构改变和终末分化的影响，鉴定其对人成骨肉瘤细胞终末分化的诱导作用。方法以中药有效成分组合（33mg／L人参皂甙Rg1＋2mmol／L肉桂酸＋0．3mg／L丹参酮ⅡA）处理MG-63细胞7d，对照组与处理组样本各3次重复，免疫细胞化学检测及光镜与电镜观察系统研究RCT组合对MG-63细胞的细胞生物学效应。环六亚甲基双乙酰胺（HMBA）处理MG-63细胞作为分化诱导效果的阳性对照。结果免疫细胞化学检测显示，成骨肉瘤细胞终末分化指标I型胶原、骨黏素、骨钙蛋白的表达呈强阳性，并观察到钙化糖原颗粒增多与典型骨结节的形成；光镜与电镜观察结果显示，处理后的MG-63细胞产生了细胞形态规则，大小-致，细胞铺展体积增大，核质比例减小，核内核仁数目减少，细胞器丰富发达等趋向正常细胞特征的显著改变。结论RCT组合能显著改变MG．63细胞形态与超微结构恶性特征，并促进终末分化标志物的表达，从而对人成骨肉瘤MG-63细胞的终末分化具有显著诱导作用。Objective This study explored the effects of the effective component of Chinese herbs on the morphology, uhrastructure and terminal differentiation phenotype of human osteosareoma, and in addition identified its inducing function of terminal differentiation of MG-63 cell. Methods MG-63 cells were treated with the combination of 33 mg/L ginsenoside Rgl, 2mmol/L cinnamic acid and 0.3 mg/L tanshinonelIA （RCT） for 7 days and three samples of each groups were subjected to immuncocytochemical assay,and light and electro-microscopy. The MG-63 cells treated by hexamethylene bisacetamide（HMBA） were investigated as a positive control of induced differentiation. Results Immunocytochemistry analysis revealed that the expression of the molecular biomarkers of terminal differentiation, including type I collagen, osteocalcin and osteonectin, were notabley positive, and more calcified glycogen particles and classic bone nodules were obsreved in the RCT treated cells. Using light and electro-microscopy, we found that the morphology and ultrastructure of MG-63 cells had undergone restorational changes after RCT treatments similar to that of normal cells ： the cells were regular and inclined to have the same size, the size increased, the nucleo-cytoplasm ratio lessened, the number and size of nucleous decreased, organelles tended to be well-developed, and were typical and polarized. Conclusion From these observations we have drawn the conclusion that RCT could reverse the malignant phenotypes of tumor cells, promote the expression of terminal differentiational biomarkers, and remarkably induced MG-63 cell terminal differentiation

关 键 词：人参皂甙RG1 肉桂酸 丹参酮ⅡA 人成骨肉瘤MG-63细胞 免疫细胞化学 电镜 

分 类 号：Q247[生物学—细胞生物学] Q254