过表达外源M-CSF促进MCF7细胞增殖  

Overexpression of M-CSF Promotes Proliferation of MCF7 Cells

作　　者：涂剑[1] 姚志红[1] 龙治峰[2] 朱炳阳[1] 唐圣松[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001 [2]南华大学组胚学教研室,湖南衡阳421001

出　　处：《中国生物化学与分子生物学报》2009年第2期132-136,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助项目(No.30270684);湖南省自然科学基金项目资助(No.08JJ5004)~~

摘　　要：为探讨过表达外源巨噬细胞集落刺激因子(M-CSF)对细胞增殖的影响,将重组载体pCMV/cyto/myc-M-CSF转染MCF7细胞、G418筛选,RT-PCR、Western印迹和免疫荧光鉴定M-CSF的mRNA表达、蛋白表达及定位,通过计算细胞倍增时间、MTT法及反义寡核苷酸抑制实验分析M-CSF对细胞增殖的影响.结果表明,转染M-CSF的MCF7细胞过表达M-CSF mRNA及蛋白,并且定位表达于细胞质;与转染空载体及未转染M-CSF组细胞比较,转染M-CSF的MCF7细胞倍增时间明显缩短、增殖能力显著增强,M-CSF的特异性反义寡核苷酸能抑制转染M-CSF的MCF7细胞的增殖,且抑制率随着反义寡核苷酸浓度的增高而增强;以上结果提示,胞质过表达M-CSF可促进MCF7细胞的增殖.To explore the effect of macrophage colony stimulating factor （M-CSF） on the cell proliferation, pCMV/cyto/myc-M-CSF vectors were transfected into MCF7 cells using liposomes and screened with G418. RT-PCR, Western blot and immunofluorescence assays were performed to verify the M-CSF mRNA or protein expressions and localization. The results indicated that a high level of M-CSF expression in the cytoplasm was detected which promoted cell proliferation as shown by cell doubling time, MTT assays in M-CSF-transfected MCF7 cells. In addition, the proliferation of MCF7 cells transfected with M-CSF-specific antisense oligonucleotides was significantly inhibited than those of pCMV/cyto/myc transfected or untransfected MCF7 cells. We conclude that the overexpression of M-CSF promotes the proliferation of MCF7 cells.

关 键 词：巨噬细胞集落刺激因子 pCMV/cyto/myc—M—CSF载体 MCF7细胞 胞质 细胞增殖 

分 类 号：Q510.4[生物学—生物化学] Q253