北京怀柔地区李矮缩病毒的检测鉴定  

Detection and identification of prune dwarf virus in Huairou district in Beijing

作　　者：赵世恒[1] 王进忠[1] 李明福[2] 李桂芬[2] 马洁[2] 勾军伟[3] 

机构地区：[1]北京农学院植物科学技术系,北京102206 [2]中国检验检疫科学研究院动植物检疫研究所,北京100024 [3]长江大学农学院植保系,武汉434025

出　　处：《中国植保导刊》2009年第2期5-7,共3页China Plant Protection

基　　金：国家“十一五”支撑计划潜在入侵物种口岸侦测技术研究(2006BAD08A13)

摘　　要：在北京怀柔地区核果类果树病毒疫情调查中,对樱桃树上有矮缩、斑驳、皱缩等症状的叶片样品,通过酶联免疫检测为阳性反应,进行RT-PCR鉴定、外壳蛋白基因扩增片段测序,发现该序列与李属坏死环斑病毒(PDV)ch株系分离物(L28145.1)序列的同源性为100%,与分离物(AF208746.1,AF208747.1,AF208743.1)序列的同源性大于97%,说明被检测样品感染有PDV。这是PDV病毒在北京地区发生的首次报道,对该病毒来源及其流行趋势也进行了分析。Some leaves with symptoms of dwarf, piebaldism and shrinkage in pest survey on stone fruit trees in Huairou district in Beijing were tested by enzyme-linked immunosorbent assay （ELISA）, which showed the positive reaction for prune dwarf virus （PDV）. And the result of RT-PCR identification and sequence analysis of amplification fragment of coat protein genome showed that homology of the sequence with the isolate of PDV-ch strain （L28145.1） was 100%, and its homology with three other isolates （AF208746.1, AF208747.1, AF208743.1） was more than 97%, which also indicated that the samples tested were infected by PDV. It was the first report of PDV occurred in Beijing and its origin and spread trend were analyzed.

关 键 词：李矮缩病毒 检测 鉴定 

分 类 号：S436.629[农业科学—农业昆虫与害虫防治]