老年支气管哮喘患者血清中白细胞介素及肿瘤坏死因子的检测及意义  

The significance of detected level of serum interleukin and TNF-α in the patients with bronchial asthma in the elderly

作　　者：曹岩[1] 黄颖[1] 钟莉莉[1] 杨春荣[2] 李方莲[3] 

机构地区：[1]吉林大学第二医院中心实验室,吉林长春130041 [2]吉林大学第二医院电诊科,吉林长春130041 [3]吉林大学第二医院药剂科,吉林长春130041

出　　处：《中国实验诊断学》2009年第2期197-199,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-8(Interleukin-8,IL-8)、白细胞介素-10(Interleukin 10,IL-10)和肿瘤坏死因子-α(Tumornecrosis factor,TNF-α)水平在支气管哮喘患者发病机制中的作用。方法选择支气管哮喘急性发作期患者组26例和缓解期患者组22例,健康人(对照组)22例的血清。采用双抗体夹心ELISA法分别测定血清中IL-4、IL-8、IL-10和TNF-α水平的变化。结果支气管哮喘急性发作期和缓解期患者血清中IL-4、IL-8、IL-10和TNF-α水平明显升高,与对照组比较差异有显著性(P<0.01),但急性发作期和缓解期患者血清中IL-8相比较无显著性差异(P>0.05)。结论测定IL-4、IL-8、IL-10和TNF-α的水平可作为判断哮喘疾病程度的重要参考指标,为探讨支气管哮喘发病机制奠定理论基础。Objective To explore the role of IL-4, IL-8, IL-10 and TNF-α in bronchial asthma patients in the pathogenesis of asthma.Methods 48 cases of asthmatic patients during exacerbation and stable stage, and 22 healthy persons were selected. The levels of IL-4, IL-8 ,IL-10 and TNF-α in serum was measured by ELISA. Results IL-4,11.-8, IL-10 and TNF-α and in the patients during exacerbation and stable stage in surum were increased significantly（ P 〈 0.01）,However, there was no marked difference between the patients with exacerbation and stable stage（ P 〉 0.05）. Conclusion It suggests that IL-4, IL-8, IL- 10 and TNF-α is a marker to judge the overall condition and severity of the disease in bronchial asthma patients.

关 键 词：老年支气管哮喘 白细胞介素 肿瘤坏死因子 

分 类 号：R562.25[医药卫生—呼吸系统]