人早孕绒毛滋养层细胞的分离纯化及马鞭草对其分泌绒毛膜促性腺激素的影响  

Trophoblast Cells Isolated from Human Villi of Early Pregnancy and HCG Secretion Effect by Verbena off cinalin L.

作　　者：徐昌芬[1] 卢晓东[1] 周亚东[1] 焦中秀[1] 祝辉[1] 朱启锭[1] 

机构地区：[1]南京医科大学组织胚胎学教研室

出　　处：《中国药科大学学报》1998年第2期145-148,共4页Journal of China Pharmaceutical University

基　　金：江苏省科委资助

摘　　要：从正常妊娠６～８周早期胎盘Ｐｅｒｃｏｌ梯度离心分离出滋养层细胞进行体外培养，观察马鞭草对培养的滋养层细胞形态及绒毛膜促性腺激素（ＨＣＧ）分泌功能的影响，探讨了马鞭草抗早孕的细胞学作用机理。结果表明２５，５０ｍｇ／ｍｌ马鞭草乙醇提取液对滋养层细胞ＨＣＧ分泌有明显的抑制作用，给药后２４ｈ即见部分细胞变圆，４８ｈ大多固缩死亡。一定浓度的马鞭草可直接杀伤滋养层细胞，抑制ＨＣＧ的分泌而中止早孕。Trophoblast cells were isolated from human villi of early pregnancy by Percoll gradient centrifugation and cultured in vitro. By observing the morphological changes of the human trophoblast cells as well as the HCG secretion,we tried to discuss the cytological mechanism of the antiearly pregnancy effect of Verbena offcinalin L. The results showed that the treated trophoblast cells had obvious morphological damage in different degrees after 24　h treatment with 25 mg/ml, 50 mg/ml Verbena offcinalin L. extract. The degrees of damage were dependent on the concentration and duration. Results proved that Verbena offcinalin L. could directly kill trophoblast cells and inhibit HCG secretion.Therefore, it can terminate the early pregnancy.

关 键 词：滋养层细胞 马鞭草 分离纯化 HCG 抗早孕 

分 类 号：R285.5[医药卫生—中药学] R286.921[医药卫生—中医学]