Cr^(6+)对加替沙星与小牛胸腺DNA相互作用的影响  

Influence of Cr^(6+) on Binding of Gatifloxacin with DNA

作　　者：秦君[1] 袁小英[1] 董川[1] 

机构地区：[1]山西大学化学化工学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2009年第1期108-110,共3页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(No.20875059)

摘　　要：通过测定Cr6+和小牛胸腺DNA对加替沙星的荧光猝灭,考察了Cr6+对加替沙星和DNA结合的影响.结果发现Cr6+和DNA对加替沙星都有不同程度的荧光猝灭作用,且Cr6+能够降低加替沙星与DNA的结合常数,原因可能是Cr6+在加替沙星与DNA的反应中对加替沙星有竞争效应,或者Cr6+与加替沙星生成了络合物.After investigating the complex reaction of gatifloxacin with Cr^6＋ and calf thymus DNA,influence of Cr^6＋ on the binding ability of gatifloxacin with DNA was analyzed. The result shows that Cr^6＋ and DNA could separately quench fluorescence of gatifloxacin; Cr^6＋ can reduce binding ability of gatifloxacin with DNA. The reasonable consideration of the results may be：Cr^6＋ act a role of competition against gatifloxacin or Cr^6＋ bind with gatifloxacin.

关 键 词：药物分析 加替沙星 荧光光谱 Cr6+ DNA 

分 类 号：O657.3[理学—分析化学]